Aquino Ana Carolina Benites, Mendes Lucas William, Pellegrinetti Thierry Alexandre, Alleoni Luís Reynaldo Ferracciú
Department of Soil Science, Luiz de Queiroz College of Agriculture, University of São Paulo (USP), Piracicaba, SP, 13418-260, Brazil.
Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture CENA, USP, Piracicaba, SP, 13416-000, Brazil.
Braz J Microbiol. 2025 May 26. doi: 10.1007/s42770-025-01695-w.
Phosphorus (P) has a strong affinity with soil colloids in humid tropical conditions, reducing its availability to plants. The use of alternative sources of P can provide nutrients to plants and reduce countries' dependence on imports of phosphate fertilizers. Some nitrogen (N) sources can acidify the soil and affect the efficiency of P fertilizers. In this study, evaluated the changes in the microbial community of the rhizosphere of maize (Zea mays) affected by N and P fertilizers in soils with contrasting textures. The N sources used were calcium nitrate (CN) and ammonium sulphate (AS), and the P sources: triple superphosphate (TSP), organomineral (OR) and struvite (ST), and two control treatments without the addition of N and P fertilizers, with (Control) and without plant (Control NP). The rhizosphere samples were subjected to genetic sequencing of the 16S rRNA region, and the structures, diversity, richness and differential abundance of the microbial communities were assessed. Distinct microbial compositions were identified between medium-textured (MT) and clayey (CT) soils, influenced by soil texture, organic matter and fertilizers. Nitrogen fertilizers had the greatest impact on the structure of microbial communities in MT soil. Differential abundance analysis revealed specific variations in microbial taxa in response to nutrient sources, with an impact on nutrient cycling and acquisition. The ST + CN treatment in MT soil was enriched with pollutant bioremediating genera such as Sphingbium, Flavitalea, Devosia and Rubellimicrobium. The study highlights the intricate interaction between soil type, fertilizer sources and microbial community dynamics, with an impact on overall productivity.
在湿润的热带条件下，磷（P）与土壤胶体具有很强的亲和力，从而降低了其对植物的有效性。使用替代磷源可以为植物提供养分，并减少各国对磷肥进口的依赖。一些氮（N）源会使土壤酸化并影响磷肥的效率。在本研究中，评估了不同质地土壤中氮磷肥对玉米（Zea mays）根际微生物群落的影响。所用的氮源为硝酸钙（CN）和硫酸铵（AS），磷源为过磷酸钙（TSP）、有机矿物肥（OR）和鸟粪石（ST），以及两种不添加氮磷肥的对照处理，一种有植物（对照），一种无植物（对照NP）。对根际样品进行16S rRNA区域的基因测序，并评估微生物群落的结构、多样性、丰富度和差异丰度。受土壤质地、有机质和肥料的影响，在中等质地（MT）土壤和黏质（CT）土壤之间鉴定出了不同的微生物组成。氮肥对MT土壤中微生物群落的结构影响最大。差异丰度分析揭示了微生物分类群对养分来源的特定变化，对养分循环和获取有影响。MT土壤中的ST + CN处理富含污染物生物修复属，如鞘氨醇单胞菌属、黄杆菌属、德沃斯氏菌属和微红菌属。该研究强调了土壤类型、肥料来源和微生物群落动态之间的复杂相互作用，对整体生产力有影响。
