Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
Ningxia Medical University, Yinchuan, China.
Clin Res Hepatol Gastroenterol. 2016 Dec;40(6):748-754. doi: 10.1016/j.clinre.2016.05.006. Epub 2016 Jun 20.
We aimed to investigate the effects of microRNA-214 (miR-214) on peritoneal metastasis as well as to elucidate its regulatory mechanism in gastric cancer (GC).
The expression levels of miR-214 in human GC cell lines MKN-28NM, MKN-28M, GC9811 and GC9811-P were analyzed by quantitative real-time PCR. Lentiviral miR-214, lentiviral miR-214 inhibitor, and empty lentiviral vector were transfected to GC cell lines, respectively. The roles of miR-214 in cell invasion, migration, proliferation and colony-forming ability were then analyzed. Besides, the expression levels of PTEN in different transfected cells were determined by western blot analysis.
We found that miR-214 was up-regulated in GC9811-P cells with high metastatic potential to the peritoneum compared with that in GC9811 cells. In addition, in vitro overexpression of miR-214 promoted cell invasion, migration, proliferation and colony-forming ability of GC9811 cells, while down-regulation of miR-214 had opposite effects in GC9811-P cells. Besides, overexpression of miR-214 in GC9811 cells markedly down-regulated PTEN expression, whereas down-regulation of miR-214 in GC9811-P cells significantly increased PTEN expression.
Our findings indicate that miR-214 may promote peritoneal metastasis of GC cells via down-regulation of PTEN, thus leading to the progression of GC.
本研究旨在探讨 microRNA-214（miR-214）对胃癌（GC）腹膜转移的影响，并阐明其在 GC 中的调控机制。
采用实时定量 PCR 分析人 GC 细胞系 MKN-28NM、MKN-28M、GC9811 和 GC9811-P 中 miR-214 的表达水平。分别转染 GC 细胞系慢病毒 miR-214、慢病毒 miR-214 抑制剂和空载慢病毒载体。然后分析 miR-214 对细胞侵袭、迁移、增殖和集落形成能力的作用。此外，通过 Western blot 分析测定不同转染细胞中 PTEN 的表达水平。
我们发现，与 GC9811 细胞相比，具有高腹膜转移潜能的 GC9811-P 细胞中 miR-214 表达上调。此外，体外过表达 miR-214 促进了 GC9811 细胞的侵袭、迁移、增殖和集落形成能力，而在 GC9811-P 细胞中下调 miR-214 则产生相反的效果。此外，在 GC9811 细胞中过表达 miR-214 显著下调了 PTEN 的表达，而在 GC9811-P 细胞中下调 miR-214 则显著增加了 PTEN 的表达。
我们的研究结果表明，miR-214 可能通过下调 PTEN 促进 GC 细胞的腹膜转移，从而导致 GC 的进展。
