微小 RNA-214 通过负向调控 PTEN 促进胃癌腹膜转移。
MicroRNA-214 promotes peritoneal metastasis through regulating PTEN negatively in gastric cancer.
机构信息
Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
Ningxia Medical University, Yinchuan, China.
出版信息
Clin Res Hepatol Gastroenterol. 2016 Dec;40(6):748-754. doi: 10.1016/j.clinre.2016.05.006. Epub 2016 Jun 20.
BACKGROUND AND OBJECTIVE
We aimed to investigate the effects of microRNA-214 (miR-214) on peritoneal metastasis as well as to elucidate its regulatory mechanism in gastric cancer (GC).
METHODS
The expression levels of miR-214 in human GC cell lines MKN-28NM, MKN-28M, GC9811 and GC9811-P were analyzed by quantitative real-time PCR. Lentiviral miR-214, lentiviral miR-214 inhibitor, and empty lentiviral vector were transfected to GC cell lines, respectively. The roles of miR-214 in cell invasion, migration, proliferation and colony-forming ability were then analyzed. Besides, the expression levels of PTEN in different transfected cells were determined by western blot analysis.
RESULTS
We found that miR-214 was up-regulated in GC9811-P cells with high metastatic potential to the peritoneum compared with that in GC9811 cells. In addition, in vitro overexpression of miR-214 promoted cell invasion, migration, proliferation and colony-forming ability of GC9811 cells, while down-regulation of miR-214 had opposite effects in GC9811-P cells. Besides, overexpression of miR-214 in GC9811 cells markedly down-regulated PTEN expression, whereas down-regulation of miR-214 in GC9811-P cells significantly increased PTEN expression.
CONCLUSIONS
Our findings indicate that miR-214 may promote peritoneal metastasis of GC cells via down-regulation of PTEN, thus leading to the progression of GC.
背景与目的
本研究旨在探讨 microRNA-214（miR-214）对胃癌（GC）腹膜转移的影响，并阐明其在 GC 中的调控机制。
方法
采用实时定量 PCR 分析人 GC 细胞系 MKN-28NM、MKN-28M、GC9811 和 GC9811-P 中 miR-214 的表达水平。分别转染 GC 细胞系慢病毒 miR-214、慢病毒 miR-214 抑制剂和空载慢病毒载体。然后分析 miR-214 对细胞侵袭、迁移、增殖和集落形成能力的作用。此外，通过 Western blot 分析测定不同转染细胞中 PTEN 的表达水平。
结果
我们发现，与 GC9811 细胞相比，具有高腹膜转移潜能的 GC9811-P 细胞中 miR-214 表达上调。此外，体外过表达 miR-214 促进了 GC9811 细胞的侵袭、迁移、增殖和集落形成能力，而在 GC9811-P 细胞中下调 miR-214 则产生相反的效果。此外，在 GC9811 细胞中过表达 miR-214 显著下调了 PTEN 的表达，而在 GC9811-P 细胞中下调 miR-214 则显著增加了 PTEN 的表达。
结论
我们的研究结果表明，miR-214 可能通过下调 PTEN 促进 GC 细胞的腹膜转移，从而导致 GC 的进展。
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