Ma Jianping, Yu Haisheng, Yin Xiangyun, Cheng Menglan, Shi Quanxing, Yin Zhao, Nie Xiaohua, Shouli Wang, Zhang Liguo
Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
University of Chinese Academy of Sciences, Beijing, 100080 China.
Biophys Rep. 2015;1:139-147. doi: 10.1007/s41048-016-0023-6. Epub 2016 Apr 11.
Plasmacytoid dendritic cells (pDCs) play important roles in initiating and regulating immune responses. pDC infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progression. However, a specific antibody for identifying pDCs by immunohistochemical staining on paraffin-embedded tissue sections is still lacking. Here, we developed a novel antibody targeted E2-2, a transcription factor preferentially expressed in pDCs. The antibody stains the nuclei of pDCs specifically in immunohistochemical analysis of various tissues from both human and rhesus monkey. This novel antibody will serve as a beneficial tool for pDC-related basic research and clinical investigation.
浆细胞样树突状细胞（pDCs）在启动和调节免疫反应中发挥着重要作用。pDC浸润已在包括感染、肿瘤和自身免疫性疾病在内的多种病理病变中得到记录，并且pDC浸润的严重程度与疾病进展相关。然而，仍然缺乏一种用于在石蜡包埋组织切片上通过免疫组织化学染色鉴定pDCs的特异性抗体。在此，我们开发了一种靶向E2-2的新型抗体，E2-2是一种在pDCs中优先表达的转录因子。在对来自人类和恒河猴的各种组织进行免疫组织化学分析时，该抗体特异性地标记pDCs的细胞核。这种新型抗体将成为pDC相关基础研究和临床研究的有益工具。
