Tanaka T, Tamatsukuri S, Ikehara M
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Nucleic Acids Res. 1987 Sep 25;15(18):7235-48. doi: 10.1093/nar/15.18.7235.
Oligoribonucleotides with chain length of 7, 11, 15, 17, 24 and 34 were synthesized on long chain alkylamine controlled pore glass beads (LCA-CPG) using o-nitrobenzyl protection of 2'-hydroxyls via a H-phosphonate approach either manually or by using an automatic synthesizer. The oligoribonucleotides were obtained in yields of 0.6 0.6-20%, based on initial nucleoside bound to the LCA-CPG support.
链长为7、11、15、17、24和34的寡核糖核苷酸是通过H-膦酸酯法，在长链烷基胺控制孔径玻璃珠（LCA-CPG）上，采用邻硝基苄基对2'-羟基进行保护，手动或使用自动合成仪合成的。基于与LCA-CPG载体结合的初始核苷，寡核糖核苷酸的产率为0.6%-20%。
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