删除肺炎克雷伯菌中的ldhA和aldH基因以提高1,3-丙二醇的产量。
Deletion of ldhA and aldH genes in Klebsiella pneumoniae to enhance 1,3-propanediol production.
作者信息
Chen Lifei, Ma Chunling, Wang Ruiming, Yang Jianlou, Zheng Haijie
机构信息
Shandong Provincial Key Laboratory of Microbial Engineering, College of Biological Engineering, Qilu University of Technology, Jinan, 250353, China.
出版信息
Biotechnol Lett. 2016 Oct;38(10):1769-74. doi: 10.1007/s10529-016-2155-7. Epub 2016 Jun 24.
OBJECTIVES
To improve 1,3-propanediol (1,3-PD) production and reduce byproduct concentration during the fermentation of Klebsiella pneumonia.
RESULTS
Klebsiella. pneumonia 2-1ΔldhA, K. pneumonia 2-1ΔaldH and K. pneumonia 2-1ΔldhAΔaldH mutant strains were obtained through deletion of the ldhA gene encoding lactate dehydrogenase required for lactate synthesis and the aldH gene encoding acetaldehyde dehydrogenase involved in the synthesis of ethanol. After fed-batch fermentation, the production of 1,3-PD from glycerol was enhanced and the concentrations of byproducts were reduced compared with the original strain K. pneumonia 2-1. The maximum yields of 1,3-PD were 85.7, 82.5 and 87.5 g/l in the respective mutant strains.
CONCLUSION
Deletion of either aldH or ldhA promoted 1,3-PD production in K. pneumonia.
目的
提高肺炎克雷伯菌发酵过程中1,3 - 丙二醇（1,3 - PD）的产量并降低副产物浓度。
结果
通过缺失编码乳酸合成所需乳酸脱氢酶的ldhA基因和参与乙醇合成的乙醛脱氢酶的aldH基因，获得了肺炎克雷伯菌2 - 1ΔldhA、肺炎克雷伯菌2 - 1ΔaldH和肺炎克雷伯菌2 - 1ΔldhAΔaldH突变株。补料分批发酵后，与原始菌株肺炎克雷伯菌2 - 1相比，甘油生成1,3 - PD的产量提高，副产物浓度降低。各突变株中1,3 - PD的最大产量分别为85.7、82.5和87.5 g/L。
结论
缺失aldH或ldhA均可促进肺炎克雷伯菌中1,3 - PD的产生。
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