Watanabe Eiki, Seike Nobuyasu, Motoki Yutaka, Inao Keiya, Otani Takashi
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan.
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan.
Ecotoxicol Environ Saf. 2016 Oct;132:288-94. doi: 10.1016/j.ecoenv.2016.06.023. Epub 2016 Jun 23.
This study evaluated the applicability of commercially available kit-based enzyme-linked immunosorbent assay (ELISA) to simple, quick, and quantitative detection for three water-extractable (phytoavailable) neonicotinoid insecticides: dinotefuran, clothianidin, and imidacloprid in soils. ELISA showed excellent analytical sensitivity for determination, but with cross-reaction to structurally related neonicotinoid analogues, which might produce false positives. To analyze insecticides in soil samples of diverse physicochemical properties, they were extracted with water. The aqueous soil extracts were assayed directly with ELISA. No matrix interference was observed without additional dilution with water. Recovery experiments for the insecticides from aqueous soil extracts spiked at 2-10 ng/mL showed good accuracy (72-126%) and precision (<16%). Kit-based ELISAs were used to estimate soil-water distribution coefficients (Kd). Values estimated using this method showed positive correlation between organic carbon contents in soil and those for evaluated insecticides. Results indicate that the evaluated kit-based ELISA has applicability for simple, quick, and reliable detection of phytoavailable insecticides in soils and for estimating Kd values in soil.
本研究评估了市售基于试剂盒的酶联免疫吸附测定法（ELISA）用于简单、快速和定量检测土壤中三种可水提取（植物可利用）的新烟碱类杀虫剂：呋虫胺、噻虫胺和吡虫啉的适用性。ELISA在测定方面表现出出色的分析灵敏度，但对结构相关的新烟碱类似物有交叉反应，这可能会产生假阳性。为了分析不同理化性质土壤样品中的杀虫剂，用水对其进行提取。土壤水提取物直接用ELISA进行检测。在不额外用水稀释的情况下未观察到基质干扰。对添加浓度为2 - 10 ng/mL的土壤水提取物中的杀虫剂进行的回收率实验显示出良好的准确度（72 - 126%）和精密度（<16%）。基于试剂盒的ELISA用于估算土壤 - 水分配系数（Kd）。用该方法估算的值显示土壤中有机碳含量与所评估杀虫剂的有机碳含量之间呈正相关。结果表明，所评估的基于试剂盒的ELISA适用于简单、快速且可靠地检测土壤中植物可利用的杀虫剂以及估算土壤中的Kd值。
