Xu Ting, Wei Ke-yi, Wang Jia, Ma Hui-xin, Li Ji, Xu Yan-jun, Li Qing X
China Agricultural University, College of Resources and Environmental Sciences, Beijing 100193, People's Republic of China.
J AOAC Int. 2010 Jan-Feb;93(1):12-8.
Rapid and sensitive ELISAs have been applied to analyze imidacloprid and thiamethoxam residues in apple, grape, orange, and peach juices after simple dilution of the samples without any extraction and cleanup. The matrix interference of the fruit juices was eliminated after 100-fold dilution, and the ELISAs gave method LODs of imidacloprid and thiamethoxam in fruit juices down to 20 and 5 ng/g, respectively. Average recoveries of imidacloprid and thiamethoxam from the spiked fruit juices were in the range of 92-115% and 87-118%, respectively. The CVs of the recoveries ranged from 3 to 19% and from 6 to 19% for imidacloprid and thiamethoxam, respectively. The ELISA results were comparable to those obtained by a reference HPLC/MS method using pesticide-spiked samples, with reasonable correlation coefficients between 0.89 and 0.93 and regression coefficients (slopes) between 0.75 and 0.94.
快速灵敏的酶联免疫吸附测定法（ELISA）已被用于分析苹果汁、葡萄汁、橙汁和桃汁中的吡虫啉和噻虫嗪残留，只需对样品进行简单稀释，无需任何提取和净化步骤。果汁样品经100倍稀释后，基质干扰被消除，ELISA法测定果汁中吡虫啉和噻虫嗪的方法检出限分别低至20 ng/g和5 ng/g。加标果汁中吡虫啉和噻虫嗪的平均回收率分别在92% - 115%和87% - 118%范围内。吡虫啉和噻虫嗪回收率的变异系数分别为3% - 19%和6% - 19%。ELISA结果与使用加标农药样品的参考高效液相色谱/质谱法（HPLC/MS）所得结果相当，相关系数在0.89至0.93之间，回归系数（斜率）在0.75至0.94之间。
