Miere Cristian, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko
Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
Stem Cell Res. 2016 Jan;16(1):17-9. doi: 10.1016/j.scr.2015.11.007. Epub 2015 Nov 26.
The KCL032 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.
KCL032人胚胎干细胞系源自一个捐赠用于研究的正常健康囊胚。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。干细胞系的建立及细胞系的传代培养均在无动物源产品的环境中按照现行药品生产质量管理规范（cGMP）标准进行。通过体外试验确认了其多能状态和分化潜能。
