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携带HBB基因突变的KCL035研究级人胚胎干细胞系的产生。

Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene.

作者信息

Hewitson Heema, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko

机构信息

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

出版信息

Stem Cell Res. 2016 Mar;16(2):210-2. doi: 10.1016/j.scr.2016.01.007. Epub 2016 Jan 14.

DOI:10.1016/j.scr.2016.01.007
PMID:27345970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4823668/
Abstract

The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

摘要

KCL035人胚胎干细胞系源自一个捐赠用于研究的胚胎，该胚胎携带与β地中海贫血综合征相关的HBB基因突变。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。细胞系的建立和传代培养均在无动物源产品的环境中进行。通过体外试验证实了其多能状态和分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/67e143275dcb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/70cec9885c85/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/16e302a0b73d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/67e143275dcb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/70cec9885c85/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/16e302a0b73d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f518/4823668/67e143275dcb/gr3.jpg

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本文引用的文献

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Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment.在无动物产品环境中从冷冻胚胎中衍生和增殖人类胚胎干细胞系。
Nat Protoc. 2012 Jun 21;7(7):1366-81. doi: 10.1038/nprot.2012.080.
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Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions.无饲养层且无异源条件下人类胚胎干细胞的分离和扩增。
Cytotherapy. 2012 Jan;14(1):122-8. doi: 10.3109/14653249.2011.623692. Epub 2011 Oct 27.
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Effect of karyotype on successful human embryonic stem cell derivation.
核型对人类胚胎干细胞成功分离的影响。
Stem Cells Dev. 2010 Jan;19(1):39-46. doi: 10.1089/scd.2009.0136.
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Derivation of hESC from intact blastocysts.从完整囊胚中获取人胚胎干细胞。
Curr Protoc Stem Cell Biol. 2007 Jun;Chapter 1:Unit 1A.2. doi: 10.1002/9780470151808.sc01a02s1.