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携带HTT基因突变的KCL013研究级人胚胎干细胞系的产生。

Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene.

作者信息

Jacquet Laureen, Hewitson Heema, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko

机构信息

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys'' Hospital, London, United Kingdom.

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys'' Hospital, London, United Kingdom.

出版信息

Stem Cell Res. 2016 Mar;16(2):293-5. doi: 10.1016/j.scr.2016.01.011. Epub 2016 Jan 14.

DOI:10.1016/j.scr.2016.01.011
PMID:27345987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4823765/
Abstract

The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

摘要

KCL013人胚胎干细胞系源自一个捐赠用于研究的胚胎，该胚胎携带一种常染色体显性突变，影响编码亨廷顿蛋白的HTT基因的一个等位基因（42个三核苷酸重复序列；正常等位基因为17个）。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。干细胞系的建立和细胞系增殖均在无动物源产品的环境中进行。通过体外试验确认了其多能状态和分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/d4e46d881075/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/b0a16ba39ada/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/2eedba837d17/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/0c681bea3623/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/d4e46d881075/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/b0a16ba39ada/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/2eedba837d17/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/0c681bea3623/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a903/4823765/d4e46d881075/gr4.jpg

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本文引用的文献

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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells.人胚胎干细胞和诱导多能干细胞构建的功能性表皮渗透屏障的 3D 体外模型。
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Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions.
无饲养层且无异源条件下人类胚胎干细胞的分离和扩增。
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Effect of karyotype on successful human embryonic stem cell derivation.核型对人类胚胎干细胞成功分离的影响。
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Derivation of hESC from intact blastocysts.从完整囊胚中获取人胚胎干细胞。
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