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携带NF1基因变异的KCL025研究级人胚胎干细胞系的产生。

Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene.

作者信息

Hewitson Heema, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko

机构信息

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United Kingdom.

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy's Hospital, London, United Kingdom.

出版信息

Stem Cell Res. 2016 Mar;16(2):256-8. doi: 10.1016/j.scr.2016.01.009. Epub 2016 Jan 14.

DOI:10.1016/j.scr.2016.01.009
PMID:27345978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4823762/
Abstract

The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

摘要

KCL025人胚胎干细胞系源自一个捐赠用于研究的胚胎，该胚胎在编码神经纤维瘤蛋白的NF1基因中携带常染色体显性突变（c.3739 - 3742 ΔTTTG）。该基因的突变与1型神经纤维瘤病、青少年粒单核细胞白血病和沃森综合征有关。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。细胞系的建立和增殖均在无动物产品的环境中进行。通过体外试验确认了多能状态和分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/6377d56580b7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/4f13163bf46b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/daad4d75288e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/6377d56580b7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/4f13163bf46b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/daad4d75288e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/275e/4823762/6377d56580b7/gr3.jpg

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本文引用的文献

1
3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells.人胚胎干细胞和诱导多能干细胞构建的功能性表皮渗透屏障的 3D 体外模型。
Stem Cell Reports. 2014 Apr 24;2(5):675-89. doi: 10.1016/j.stemcr.2014.03.009. eCollection 2014 May 6.
2
Strategy for the creation of clinical grade hESC line banks that HLA-match a target population.创建与目标人群 HLA 匹配的临床级 hESC 系银行的策略。
EMBO Mol Med. 2013 Jan;5(1):10-7. doi: 10.1002/emmm.201201973. Epub 2012 Nov 19.
3
Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment.
在无动物产品环境中从冷冻胚胎中衍生和增殖人类胚胎干细胞系。
Nat Protoc. 2012 Jun 21;7(7):1366-81. doi: 10.1038/nprot.2012.080.
4
Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions.无饲养层且无异源条件下人类胚胎干细胞的分离和扩增。
Cytotherapy. 2012 Jan;14(1):122-8. doi: 10.3109/14653249.2011.623692. Epub 2011 Oct 27.
5
Effect of karyotype on successful human embryonic stem cell derivation.核型对人类胚胎干细胞成功分离的影响。
Stem Cells Dev. 2010 Jan;19(1):39-46. doi: 10.1089/scd.2009.0136.
6
Derivation of hESC from intact blastocysts.从完整囊胚中获取人胚胎干细胞。
Curr Protoc Stem Cell Biol. 2007 Jun;Chapter 1:Unit 1A.2. doi: 10.1002/9780470151808.sc01a02s1.