为大熊猫的主食竹缺苞箭竹（禾本科）开发微卫星标记。
Development of microsatellite markers for Fargesia denudata (Poaceae), the staple-food bamboo of the giant panda.
作者信息
Lv Yan, Yu Tao, Lu Sihai, Tian Cheng, Li Junqing, Du Fang K
机构信息
College of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.
College of Life Science, Lanzhou University, Lanzhou 730000, Gansu, People's Republic of China.
出版信息
Appl Plant Sci. 2016 Jun 1;4(6). doi: 10.3732/apps.1600005. eCollection 2016 Jun.
PREMISE OF THE STUDY
There is a need for microsatellite primers to analyze genetic parameters of Fargesia denudata (Poaceae), the staple-food bamboo of the giant panda (Ailuropoda melanoleuca).
METHODS AND RESULTS
Using next-generation sequencing technology, we obtained a 75-Mb assembled sequence of F. denudata and identified 182 microsatellites. Primer pairs for 70 candidate microsatellite markers were selected and validated in four individuals, and 42 primer pairs generated reliable amplicons. Fourteen of 16 tested markers were found to be polymorphic in 72 individuals from four F. denudata populations. The number of alleles ranged from two to 19 per locus; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.87, respectively. The transferability of these 16 novel microsatellite markers was validated in five related species.
CONCLUSIONS
These markers will be useful for examining the genetic diversity, genetic structure, and cloning of F. denudata, the staple-food bamboo of the giant panda, and related bamboo species.
研究前提
需要微卫星引物来分析缺苞箭竹（禾本科）的遗传参数，缺苞箭竹是大熊猫（Ailuropoda melanoleuca）的主要食物来源。
方法与结果
利用新一代测序技术，我们获得了缺苞箭竹75兆碱基的组装序列，并鉴定出182个微卫星。从70个候选微卫星标记中选择引物对，并在4个个体中进行验证，42对引物产生了可靠的扩增子。在来自4个缺苞箭竹种群的72个个体中，16个测试标记中的14个被发现具有多态性。每个位点的等位基因数从2到19不等；观察到的杂合度和预期杂合度分别从0到1和从0到0.87不等。这16个新型微卫星标记在5个相关物种中的转移性得到了验证。
结论
这些标记将有助于研究大熊猫的主要食物来源缺苞箭竹以及相关竹种的遗传多样性、遗传结构和克隆。
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