十六个新的微卫星标记开发用于巨龙竹（禾本科），世界上最强劲的木质竹子。

Sixteen novel microsatellite markers developed for Dendrocalamus sinicus (Poaceae), the strongest woody bamboo in the world.

机构信息

Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, People's Republic of China.

出版信息

Am J Bot. 2012 Sep;99(9):e347-9. doi: 10.3732/ajb.1200029. Epub 2012 Aug 29.

DOI:10.3732/ajb.1200029

PMID:22933358

Abstract

PREMISE OF THE STUDY

Microsatellite primers were developed in the strongest and most economically important bamboo species, Dendrocalamus sinicus, to investigate its genetic variability.

METHODS AND RESULTS

Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, 16 polymorphic primer sets were identified within 24 individuals from two geographic locations. The number of alleles per locus ranged from two to four, with a mean of 2.6. The observed and expected heterozygosities varied from 0 to 1.000 and from 0.311 to 0.754, respectively. The cross-amplification of the 16 primer pairs was tested in four other Dendrocalamus species, and nine to 13 (56.3-81.3%) primer pairs were successfully amplified.

CONCLUSIONS

These markers will be useful for studies on the genetic diversity of D. sinicus, which may facilitate conservation of this species.

摘要

研究前提

在最强劲和最具经济重要性的竹种，巨龙竹（Dendrocalamus sinicus）中开发微卫星引物，以研究其遗传变异性。

方法和结果

使用序列中重复的快速 AFLP 分离（FIASCO）协议，从两个地理位置的 24 个个体中鉴定出 16 个多态性引物对。每个位点的等位基因数范围从两个到四个，平均值为 2.6。观察到的和预期的杂合度分别从 0 到 1.000 和从 0.311 到 0.754 变化。在其他四个巨龙竹种中测试了 16 对引物的交叉扩增，其中 9 到 13 对（56.3-81.3%）引物对成功扩增。