开发琴叶珊瑚（红树科）微卫星标记。
Development of microsatellite markers for Carallia brachiata (Rhizophoraceae).
机构信息
Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
South China Agricultural University, Guangzhou 510642, Guangdong, People's Republic of China.
出版信息
Appl Plant Sci. 2015 Mar 9;3(3). doi: 10.3732/apps.1400125. eCollection 2015 Mar.
PREMISE OF THE STUDY
Microsatellite markers were developed for Carallia brachiata to assess the genetic diversity and structure of this terrestrial species of the Rhizophoraceae.
METHODS AND RESULTS
Based on transcriptome data for C. brachiata, 40 primer pairs were initially designed and tested, of which 18 were successfully amplified and 11 were polymorphic. For these microsatellites, one to three alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 0.727 and 0 to 0.520, respectively. In addition, all primers were successfully amplified in two congeners: C. pectinifolia and C. garciniifolia.
CONCLUSIONS
The microsatellite markers described here will be useful in population genetic studies of C. brachiata and related species, suggesting that developing microsatellite markers from next-generation sequencing data can be efficient for genetic studies across this genus.
研究前提
为 Carallia brachiata 开发了微卫星标记，以评估这种 Rhizophoraceae 陆生物种的遗传多样性和结构。
方法和结果
基于 C. brachiata 的转录组数据，最初设计和测试了 40 对引物，其中 18 对成功扩增，11 对多态性。对于这些微卫星，每个位点可识别一到三个等位基因。观察到的和预期的杂合度分别在 0 到 0.727 和 0 到 0.520 之间。此外，所有引物在两个近缘种中均成功扩增：C. pectinifolia 和 C. garciniifolia。
结论
此处描述的微卫星标记将有助于对 C. brachiata 和相关物种的种群遗传研究，表明从下一代测序数据开发微卫星标记可有效地用于该属的遗传研究。
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