Jin Decai, Kong Xiao, Liu Huijun, Wang Xinxin, Deng Ye, Jia Minghong, Yu Xiangyang
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Beijing University of Agriculture, Beijing 102206, China.
Int J Mol Sci. 2016 Jun 25;17(7):1012. doi: 10.3390/ijms17071012.
A bacterial strain QH-12 isolated from activated sludge was identified as Gordonia sp. based on analysis of 16S rRNA gene sequence and was found to be capable of utilizing dibutyl phthalate (DBP) and other common phthalate esters (PAEs) as the sole carbon and energy source. The degradation kinetics of DBP under different concentrations by the strain QH-12 fit well with the modified Gompertz model (R² > 0.98). However, strain QH-12 could not utilize the major intermediate product phthalate (phthalic acid; PA) as the sole carbon and energy source, and only a little amount of PA was detected. The QH-12 genome analysis revealed the presence of putative hydrolase/esterase genes involved in PAEs-degradation but no phthalic acid catabolic gene cluster was found, suggesting that a novel degradation pathway of PAEs was present in Gordonia sp. QH-12. This information will be valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs-degrading Gordonia sp. strains.
从活性污泥中分离出的一株细菌QH-12，基于16S rRNA基因序列分析被鉴定为戈登氏菌属，并且发现它能够利用邻苯二甲酸二丁酯（DBP）和其他常见的邻苯二甲酸酯（PAEs）作为唯一的碳源和能源。菌株QH-12在不同浓度下对DBP的降解动力学与修正的Gompertz模型拟合良好（R²>0.98）。然而，菌株QH-12不能利用主要中间产物邻苯二甲酸（邻苯二甲酸；PA）作为唯一的碳源和能源，仅检测到少量的PA。QH-12基因组分析揭示了存在与PAEs降解相关的假定水解酶/酯酶基因，但未发现邻苯二甲酸分解代谢基因簇，这表明戈登氏菌属QH-12中存在一种新的PAEs降解途径。这些信息对于更全面地了解PAEs降解戈登氏菌属菌株的多种遗传机制具有重要价值。
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