Lee Jeong-Ho, Lim Jeong-Muk, Velmurugan Palanivel, Park Yool-Jin, Park Youn-Jong, Bang Keuk-Soo, Oh Byung-Taek
Sunchang Research Institute of Health and Longevity, Sunchang, Jeonbuk 56015, South Korea.
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 54596, South Korea.
J Photochem Photobiol B. 2016 Sep;162:93-99. doi: 10.1016/j.jphotobiol.2016.06.029. Epub 2016 Jun 23.
We present the simple, eco-friendly synthesis of silver nanoparticles (AgNPs) using sunlight or green, red, blue, or white LED light together with Dryopteris crassirhizoma rhizome extract (DCRE) as the reducing and capping agent. The preliminary indication of AgNP production was a color change from yellowish green to brown after light exposure in the presence of DCRE. Optimization of parameters such as pH, inoculum dose, and metal ion concentration played an important role in achieving nanoparticle production in 30min. The spectroscopic and morphological properties of AgNPs were characterized using UV-Vis spectroscopy through the presence of a characteristic surface plasmon resonance (SPR) band for AgNPs, Fourier transform infrared spectroscopy (FT-IR), high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction (XRD). The FT-IR results indicated that the phytochemical present in DCRE was the probable reducing/capping agent involved in the synthesis of AgNPs, and light radiation enhanced nanoparticle production. HR-TEM revealed that the AgNPs were almost spherical with an average size of 5-60nm under all light sources. XRD studies confirmed the face cubic center (fcc) unit cell structure of AgNPs. The synthesized AgNPs showed good antimicrobial activity against Bacillus cereus and Pseudomonas aeruginosa. This study will bring a new insight in ecofriendly production of metal nanoparticles.
我们展示了一种简单、环保的银纳米颗粒（AgNPs）合成方法，该方法使用阳光或绿色、红色、蓝色或白色LED光，同时以粗茎鳞毛蕨根茎提取物（DCRE）作为还原剂和封端剂。在DCRE存在的情况下，光照后AgNP产生的初步迹象是颜色从黄绿色变为棕色。pH值、接种剂量和金属离子浓度等参数的优化在30分钟内实现纳米颗粒的产生中起着重要作用。通过AgNPs特征性表面等离子体共振（SPR）带的存在，利用紫外-可见光谱、傅里叶变换红外光谱（FT-IR）、高分辨率透射电子显微镜（HR-TEM）和X射线衍射（XRD）对AgNPs的光谱和形态特性进行了表征。FT-IR结果表明，DCRE中存在的植物化学物质可能是参与AgNPs合成的还原剂/封端剂，光辐射增强了纳米颗粒的产生。HR-TEM显示，在所有光源下，AgNPs几乎呈球形，平均尺寸为5-60nm。XRD研究证实了AgNPs的面心立方（fcc）晶胞结构。合成的AgNPs对蜡样芽孢杆菌和铜绿假单胞菌表现出良好的抗菌活性。这项研究将为金属纳米颗粒的绿色生产带来新的见解。
