无花果蛋白酶对2型糖尿病大鼠的抗氧化、抗血脂及抗糖尿病作用及其对葡萄糖转运蛋白4（GLUT4）转位和过氧化物酶体增殖物激活受体γ（PPARγ）表达的影响
Antioxidant, antilipidemic and antidiabetic effects of ficusin with their effects on GLUT4 translocation and PPARγ expression in type 2 diabetic rats.
作者信息
Irudayaraj Santiagu Stephen, Stalin Antony, Sunil Christudas, Duraipandiyan Veeramuthu, Al-Dhabi Naif Abdullah, Ignacimuthu Savarimuthu
机构信息
Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600 034, India.
Division of Bioinformatics, Entomology Research Institute, Loyola College, Chennai 600 034, India.
出版信息
Chem Biol Interact. 2016 Aug 25;256:85-93. doi: 10.1016/j.cbi.2016.06.023. Epub 2016 Jun 24.
In this study, the antioxidant, antilipidemic and antidiabetic effects of ficusin isolated from Ficus carica leaves and their effects on GLUT4 translocation and PPARγ expression were evaluated in HFD-STZ induced type 2 diabetic rats. Ficusin (20 and 40 mg/kg b. wt.) lowered the levels of fasting blood glucose, plasma insulin and body weight gain, in HFD-STZ induced diabetic rats. Ficusin also significantly lowered the serum antioxidant enzymes (SOD, CAT and GPx) and lipids (TC, TG and FFA) levels to near normal. Ficusin significantly enhanced the PPARγ expression and improved the translocation and activation of GLUT4 in the adipose tissue. Molecular docking analysis exhibited promising interactions of GLUT4 and PPARγ into their active sites. This study suggests that ficusin improved the insulin sensitivity on adipose tissue and it can be used for the treatment of obesity related type 2 diabetes mellitus.
在本研究中，对从无花果叶中分离出的无花果蛋白酶的抗氧化、抗血脂和抗糖尿病作用及其对HFD-STZ诱导的2型糖尿病大鼠中GLUT4转位和PPARγ表达的影响进行了评估。在HFD-STZ诱导的糖尿病大鼠中，无花果蛋白酶（20和40mg/kg体重）降低了空腹血糖、血浆胰岛素水平和体重增加。无花果蛋白酶还显著降低了血清抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）和脂质（总胆固醇、甘油三酯和游离脂肪酸）水平至接近正常。无花果蛋白酶显著增强了PPARγ的表达，并改善了脂肪组织中GLUT4的转位和激活。分子对接分析显示GLUT4和PPARγ在其活性位点有良好的相互作用。本研究表明，无花果蛋白酶改善了脂肪组织的胰岛素敏感性，可用于治疗肥胖相关的2型糖尿病。
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