Wu Tao, Xiong Lu, Wang Fuli, Xu Xiaozhen, Wang Jiao, Lin Feng, Li Chunhua, Lu Ling, Zhou Yuanping
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Cell Physiol Biochem. 2016;39(1):316-30. doi: 10.1159/000445626. Epub 2016 Jun 29.
BACKGROUND/AIMS: Different genotypes of HCV may differ in both disease progression and response to antiviral therapies. Hainan Island has been inhabited by the "Li" aboriginal minority for centuries. We aimed to provide a better understanding of HCV infection on Hainan Island, so that the information would help improve strategies for HCV prevention and control on the island and in the wider country.
Using RT-PCR and DNA sequencing, we determined HCV sequences from 100 patients living on Hainan Island.
Phylogenetic analysis classified these sequences into six subtypes: 6a (n=35), 1b (n=31), 3b (n=16), 2a (n=8), 3a (n=6), and 1a (n=4). By including reference sequences reported from elsewhere in China, phylogeographic trees were reconstructed to indicate their migration patterns. While the predominant 6a isolates were estimated to have origins in Guangdong and Guangxi provinces, the increase in 3b strains must have resulted from IDU network transmission from the southwest. A Bayesian Skyline Plot for subtype 1a, which is rare in China, showed a rapid population growth since 1998. Although slowed in rate around 2005, this growth continued to the present. Not found for any other HCV lineage.
Overall, a delayed growth pattern may indicate the unique history of 1a dissemination in China and its recently increasing prevalence, despite measures taken to improve HCV prevention.
背景/目的：丙型肝炎病毒（HCV）的不同基因型在疾病进展和对抗病毒治疗的反应方面可能存在差异。海南岛数百年来一直居住着少数民族“黎族”。我们旨在更好地了解海南岛的HCV感染情况，以便这些信息有助于改进该岛及更广泛地区的HCV预防和控制策略。
我们使用逆转录聚合酶链反应（RT-PCR）和DNA测序技术，确定了100名居住在海南岛患者的HCV序列。
系统发育分析将这些序列分为六个亚型：6a（n = 35）、1b（n = 31）、3b（n = 16）、2a（n = 8）、3a（n = 6）和1a（n = 4）。通过纳入中国其他地区报道的参考序列，重建了系统发育地理树以表明其迁移模式。虽然估计占主导地位的6a分离株起源于广东省和广西壮族自治区，但3b毒株的增加必定是由于来自西南部的注射吸毒网络传播所致。中国罕见的1a亚型的贝叶斯天际线图显示，自1998年以来其种群迅速增长。尽管在2005年左右增速放缓，但这种增长一直持续到现在。其他HCV谱系未发现这种情况。
总体而言，增长延迟模式可能表明1a亚型在中国传播的独特历史及其近期患病率的上升，尽管已采取措施改善HCV预防。
