Guangzhou Blood Center, Guangzhou, China.
J Viral Hepat. 2011 Jan;18(1):42-52. doi: 10.1111/j.1365-2893.2010.01280.x.
Recently, we studied hepatitis C virus (HCV) sera-prevalence among 559 890 first-time volunteer blood donors in China. From randomly selected 450 anti-HCV positive donors, we detected HCV RNA in 270 donors. In this study, we amplified HCV E1 and/or NS5B sequences from 236 of these donors followed by DNA sequencing and phylogenetic analysis. The results indicate new trends of HCV infection in China. The HCV genotype distribution differed according to the donors' region of origin. Among donors from Guangdong province, we detected subtypes 6a, 1b, 3a, 3b, 2a, and 1a at frequencies of 49.7%, 31.0%, 7.6%, 5.5%, 4.1%, and 2.1%, respectively. Among donors from outside Guangdong, we detected 1b, 2a, 6a, 3b, 3a, 6e, and 6n at frequencies 57.1%, 13.2%, 11.0%, 9.9%, 4.4%, 2.2%, and 2.2%, respectively. Although we found no significant differences among regions in age or gender, subtype 6a was more common (P < 0.001) in donors from Guangdong than those from elsewhere, whilst subtypes 1b (P < 0.02) and 2a (P < 0.001) were more frequent outside Guangdong. Disregarding origins, the male/female ratio was higher for subtype 6a-infected donors (P < 0.05) than for subtype 1b donors, whilst the mean age of subtype 2a donors was 8-10 years older (P < 0.05) than that for all other subtypes. Detailed phylogenetic analysis of our sequence data provides further insight into the transmission of HCV within China, and between China and other countries. The predominance of HCV 6a among blood donors in Guangdong is striking and mandates studies into risk factors for its acquisition.
最近，我们研究了中国 559890 名首次献血志愿者中的丙型肝炎病毒（HCV）血清流行率。从随机选择的 450 名抗-HCV 阳性供者中，我们在 270 名供者中检测到 HCV RNA。在这项研究中，我们从 236 名供者中扩增了 HCV E1 和/或 NS5B 序列，随后进行 DNA 测序和系统发育分析。结果表明，中国 HCV 感染出现了新趋势。HCV 基因型分布因供者原籍地区而异。在来自广东省的供者中，我们检测到亚型 6a、1b、3a、3b、2a 和 1a 的频率分别为 49.7%、31.0%、7.6%、5.5%、4.1%和 2.1%。在来自广东省以外的供者中，我们检测到 1b、2a、6a、3b、3a、6e 和 6n 的频率分别为 57.1%、13.2%、11.0%、9.9%、4.4%、2.2%和 2.2%。尽管我们没有发现不同地区在年龄或性别方面存在显著差异，但在来自广东的供者中，6a 亚型更为常见（P<0.001），而在来自广东以外的供者中，1b 亚型（P<0.02）和 2a 亚型（P<0.001）更为常见。不考虑来源，6a 亚型感染者的男性/女性比例高于 1b 亚型（P<0.05），而 2a 亚型供者的平均年龄比其他所有亚型大 8-10 岁（P<0.05）。对我们序列数据的详细系统发育分析进一步深入了解了中国境内以及中国与其他国家之间 HCV 的传播情况。广东献血者中 HCV 6a 的流行率很高，这很引人注目，需要研究其获得的危险因素。
