Li Qiongfen, Yao Yufeng, Shen Yunsong, Cao Danfeng, Li Yalin, Zhang Shuqiong, Cun Wei, Sun Mingbo, Yu Jiankun, Shi Li, Dong Shaozhong
Division for Expanded Program of Immunization of Yunnan, Center for Disease Control and Prevention, Kunming, 650011, China.
Institute of Medical Biology, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
Virus Genes. 2017 Apr;53(2):190-196. doi: 10.1007/s11262-016-1420-0. Epub 2016 Dec 23.
Recently, we reported that the frequency of hepatitis C virus (HCV) genotypes and subtypes has rapidly changed among intravenous drug users (IDUs) in Yunnan Province over the last 5 years; this is especially true for subtype 6a which has increased in frequency from 5 to 15%. Here, we assessed 120 HCV-positive plasma samples from the general population (GP). HCV NS5B fragments were amplified and sequenced by PCR. We identified four HCV genotypes (1, 2, 3 and 6) and seven HCV subtypes (1b, 2a, 3a, 3b, 6a, 6n, and 6k) in this population. Genotype 3 was predominant, with a distribution frequency of 0.484, followed by genotype 1 (0.283), genotype 6 (0.133) and genotype 2 (0.100). HCV subtypes 3b (frequency 0.292) and 1b (frequency 0.283) were the most common subtypes. A comparison of the current data with previous results reported for IDUs showed that the distribution frequencies of genotypes 1, 2 and 6 were significantly different between patients in the GP and IDUs (P < 0.05). Among the HCV subtypes, the distribution frequencies of 1b, 2a, 6a, and 6n were significantly different between patients in the GP and IDU groups (P < 0.05). Moreover, Phylogenetic analyses showed that HCV subtype 6a strains isolated from IDUs and the GP were intermixed and not separately clustered. HCV subtype 6a was predominant not only among IDUs but also among those in the GP in the Guangdong Province and Vietnam. However, HCV subtype 6a was predominant only among IDUs and not among those in the GP in the Yunnan and Guangxi Provinces. Our results indicate that the HCV subtype 6a could rapidly spread across China.
最近，我们报道了过去5年中云南省静脉吸毒者（IDU）丙型肝炎病毒（HCV）基因型和亚型的频率迅速变化；6a亚型尤其如此，其频率从5%增加到了15%。在此，我们评估了120份来自普通人群（GP）的HCV阳性血浆样本。通过PCR扩增并测序HCV NS5B片段。我们在该人群中鉴定出4种HCV基因型（1、2、3和6）和7种HCV亚型（1b、2a、3a、3b、6a、6n和6k）。3型基因型占主导，分布频率为0.484，其次是1型基因型（0.283）、6型基因型（0.133）和2型基因型（0.100）。HCV 3b亚型（频率0.292）和1b亚型（频率0.283）是最常见的亚型。将当前数据与先前报道的IDU结果进行比较显示，普通人群和IDU患者中1、2和6型基因型的分布频率存在显著差异（P < 0.05）。在HCV亚型中，普通人群和IDU组患者中1b、2a、6a和6n的分布频率存在显著差异（P < 0.05）。此外，系统发育分析表明，从IDU和普通人群中分离出的HCV 6a亚型菌株相互混杂，没有单独聚类。HCV 6a亚型不仅在IDU中占主导，在广东省和越南的普通人群中也占主导。然而，HCV 6a亚型仅在IDU中占主导，在云南和广西的普通人群中并非如此。我们的结果表明，HCV 6a亚型可能在中国迅速传播。
