Abdelraheem Wedad M, Hassuna Noha A, Abuloyoun Sahar M, Abdel Ghany Hend M, Rizk Hazem A, Abdelwahab Sayed F
Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, 61511, Egypt.
Biochemistry Department, Faculty of Medicine, Minia University, Minia, 61511, Egypt.
Arch Virol. 2016 Sep;161(9):2473-80. doi: 10.1007/s00705-016-2948-y. Epub 2016 Jun 28.
A single-nucleotide polymorphism (SNP) in the interleukin (IL)-28B gene was used as a major predictor of the response to treatment in patients with hepatitis C virus (HCV) infection. Data examining the role of IL-10 and IL-18 gene polymorphisms among HCV genotype 4 (G4)-infected Egyptians in response to pegylated interferon (PEG-IFN) plus ribavirin (RBV) therapy are limited. This study investigated the impact of SNP at IL-10.rs1800896 (at position -1082) and IL-18.rs1946518 genes (at position -607) on the response to PEG-IFN/RBV therapy in HCV-infected Egyptians. This study was carried out on 100 HCV patients treated with PEG-IFN plus RBV and 100 healthy controls. The HCV patients included 50 treatment non-responders (NR) and 50 subjects with sustained virologic response (SVR). Genomic DNA from venous blood of subjects was extracted and IL-10.rs1800896 and IL-18.rs1946518 genotypes were determined using allele-specific amplification and SYBR Green real-time PCR. Linkage disequilibrium between the two SNPs was estimated using Haploview software. The frequency of the IL-10.rs1800896 AA, AG and GG genotypes among non-responders were 16 %, 70 % and 14 % while among SVR subjects, the frequency was 34 %, 60 % and 6 %, respectively (p=0.073). On the other hand, the frequency of the IL-18.rs1946518 AA, AC and CC genotypes among non-responders was 14 %, 50 % and 36 %, respectively, while among responders, these frequencies were 28 %, 44 % and 28 %, (p = 0.220). Both markers were in linkage equilibrium (D' = 0.23; r (2) = 0.052). SNPs in the IL-10.rs1800896 and IL-18.rs1946518 genes could not predict the outcome of HCV infection in Egyptians treated with PEG-IFN/RBV.
白细胞介素（IL）-28B基因中的单核苷酸多态性（SNP）被用作丙型肝炎病毒（HCV）感染患者治疗反应的主要预测指标。关于IL-10和IL-18基因多态性在感染HCV基因4型（G4）的埃及人中对聚乙二醇干扰素（PEG-IFN）联合利巴韦林（RBV）治疗反应的作用的数据有限。本研究调查了IL-10.rs1800896（位于-1082位置）和IL-18.rs1946518基因（位于-607位置）的SNP对HCV感染的埃及人接受PEG-IFN/RBV治疗反应的影响。本研究对100例接受PEG-IFN加RBV治疗的HCV患者和100例健康对照进行。HCV患者包括50例治疗无应答者（NR）和50例持续病毒学应答（SVR）者。提取受试者静脉血中的基因组DNA，使用等位基因特异性扩增和SYBR Green实时PCR测定IL-10.rs1800896和IL-18.rs1946518基因型。使用Haploview软件估计两个SNP之间的连锁不平衡。无应答者中IL-10.rs1800896 AA、AG和GG基因型的频率分别为16%、70%和14%，而在SVR受试者中，频率分别为34%、60%和6%（p=0.073）。另一方面，无应答者中IL-18.rs1946518 AA、AC和CC基因型的频率分别为14%、50%和36%，而在应答者中，这些频率分别为28%、44%和28%（p = 0.220）。两个标记处于连锁平衡状态（D' = 0.23；r(2) = 0.052）。IL-10.rs1800896和IL-18.rs1946518基因中的SNP不能预测接受PEG-IFN/RBV治疗的埃及人HCV感染的结果。
