Wang Shao-Jie, Yin Meng-Hong, Jiang Dong, Zhang Zheng-Zheng, Qi Yan-Song, Wang Hai-Jun, Yu Jia-Kuo
1 Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital , Beijing, China .
2 Department of Joint Surgery, Zhongshan Hospital of Xiamen University, Xiamen, China .
Stem Cells Dev. 2016 Aug 15;25(16):1195-207. doi: 10.1089/scd.2016.0055. Epub 2016 Jul 27.
An increasing number of studies have detected mesenchymal stromal cells (MSCs) and mesenchymal progenitor cells (MPCs) in the peripheral blood (PB). This study aimed to systematically review the possibility of using the PB as a source for chondrogenic progenitors. PubMed, the Web of Science, and Embase were searched for relevant articles. The findings of the studies were reviewed to evaluate the biological characteristics of PB-derived MSCs, chondrogenic MPCs, and their applications in cartilage repair. Thirty-six articles were included in the final analysis, 29 of which indicated that PB is a potential source for chondrogenic progenitor cells. Thirty-two studies reporting in vitro data, including 79.2% (19/24) of studies on PB MSCs and 75% (6/8) of studies on chondrogenic PB MPCs, confirmed the existence of PB MSCs and PB MPCs, respectively; all in vivo investigations showed that using PB as a cell source enhanced cartilage repair. PB MSCs were found in most of the animal studies (12/13), whereas 7 of 11 human studies described the presence of PB MSCs. This systematic review strongly indicates the existence of MSCs in the PB of animals, whereas the presence of MSCs in human PB is less clear. Although the presence of both MSCs and chondrogenic MPCs in the PB, as well as a few favorable outcomes associated with the use of PB-derived progenitors for cartilage repair in vivo, suggests that the PB is a potential alternative source of chondrogenic progenitor cells for cartilage repair, the efficacy of these cells has not been compared to those from other sources, such as bone marrow or adipose tissue in controlled studies.
越来越多的研究在外周血（PB）中检测到间充质基质细胞（MSC）和间充质祖细胞（MPC）。本研究旨在系统评价将外周血作为软骨形成祖细胞来源的可能性。检索了PubMed、科学网和Embase以获取相关文章。对这些研究的结果进行综述，以评估外周血来源的MSC、软骨形成MPC的生物学特性及其在软骨修复中的应用。最终分析纳入了36篇文章，其中29篇表明外周血是软骨形成祖细胞的潜在来源。32项研究报告了体外数据，其中分别有79.2%（19/24）的外周血MSC研究和75%（6/8）的软骨形成外周血MPC研究证实了外周血MSC和外周血MPC的存在；所有体内研究均表明以外周血作为细胞来源可增强软骨修复。在大多数动物研究（12/13）中发现了外周血MSC，而在11项人体研究中有7项描述了外周血MSC的存在。这项系统评价有力地表明动物外周血中存在MSC，而人体外周血中MSC的存在情况尚不清楚。尽管外周血中存在MSC和软骨形成MPC，以及在体内使用外周血来源的祖细胞进行软骨修复有一些良好结果，这表明外周血是软骨修复中软骨形成祖细胞的潜在替代来源，但在对照研究中，这些细胞的疗效尚未与来自其他来源（如骨髓或脂肪组织）的细胞进行比较。
