Watabe Naoki, Joo Kim Cheol, Kimura Shunsaku
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan.
Biopolymers. 2017 Mar;108(2). doi: 10.1002/bip.22903.
Three amphiphilic block polypeptides, (sarcosine) -b-(l- or d-Leu-Aib) (L16, D16, D14), having different helical chain lengths or helicity are synthesized. A mixture of L16, D16, and D14 generates vesicles of diameters more than ca. 130 nm by injecting the ethanol solution into water and heating at 90°C for 1 h. On the other hand, when nanotubes composed of L16 and D14 having ca. 50 nm diameter are mixed with nanosheets composed of D16, smaller and homogeneous vesicles of ca. 60 nm diameter are obtained with the heat treatment. The time lapse TEM image analysis of the mixtures revealed some transient structures of nanotubes sticking a nanosheet or a vesicle at the open end of nanotubes. The precise size control of vesicles is therefore attainable by using nanotubes as a structural template regulating the size of vesicles near to the nanotube diameter upon the membrane fission processes.
合成了三种具有不同螺旋链长度或螺旋度的两亲性嵌段多肽，即（肌氨酸）-b-（l-或d-亮氨酸-氨基异丁酸）（L16、D16、D14）。通过将乙醇溶液注入水中并在90°C加热1小时，L16、D16和D14的混合物会生成直径大于约130 nm的囊泡。另一方面，当将直径约50 nm的由L16和D14组成的纳米管与由D16组成的纳米片混合时，经过热处理可得到直径约60 nm的更小且均匀的囊泡。对混合物的延时透射电子显微镜图像分析揭示了纳米管在其开口端附着纳米片或囊泡的一些瞬态结构。因此，通过使用纳米管作为结构模板，在膜裂变过程中调节囊泡大小使其接近纳米管直径，可实现对囊泡精确的尺寸控制。
