Itagaki Toru, Ueda Yuna, Itabashi Kenji, Uji Hirotaka, Kimura Shunsaku
Department of Material Chemistry Graduate School of Engineering, Kyoto University Kyoto-Daigaku-Katsura, Nishikyo-ku Kyoto 615-8510, Japan.
Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Kyoto-Daigaku-Katsura, Nishikyo-ku Kyoto 615-8510, Japan.
Chimia (Aarau). 2018 Dec 19;72(12):842-847. doi: 10.2533/chimia.2018.842.
Three kinds of amphiphilic polypeptides, X-poly(sarcosine)--(-Leu-Aib) (X = adenine, thymine, glycolic acid), were synthesized and self-assembled in a tris buffer to take on nanotube morphology. The nanotubes were joined together to extend the nanotube length with the addition of trifluoroethanol and heat treatment at 50 °C for 24 h. The length extension rate decreased in the order of adenine > glycolic acid > thymine depending on the -terminal chromophores. Adenine-adenine interactions between the nanotubes were found to be more prevalent upon joining the nanotubes than adenine-thymine interactions. Further, adenines on the nanotube surface could chelate with Cu(ii) to thermodynamically stabilize the nanotube membrane. AFM imaging in liquid environment revealed that the membrane elasticity of the adenine nanotube was as high as ca. 1 MPa, which is considered to be strengthened as a result of the adenine-adenine interactions.
合成了三种两亲性多肽，即X-聚（肌氨酸）-（-亮氨酸-氨基异丁酸）（X = 腺嘌呤、胸腺嘧啶、乙醇酸），并在三羟甲基氨基甲烷缓冲液中自组装形成纳米管形态。加入三氟乙醇并在50℃下热处理24小时后，纳米管连接在一起以延长纳米管长度。根据末端发色团的不同，长度延长率按腺嘌呤>乙醇酸>胸腺嘧啶的顺序降低。发现纳米管之间的腺嘌呤-腺嘌呤相互作用在连接纳米管时比腺嘌呤-胸腺嘧啶相互作用更普遍。此外，纳米管表面的腺嘌呤可以与Cu（II）螯合，从而在热力学上稳定纳米管膜。液体环境中的原子力显微镜成像显示，腺嘌呤纳米管的膜弹性高达约1 MPa，这被认为是腺嘌呤-腺嘌呤相互作用导致的增强结果。
