Mahayidin Hasni, Yahya Nurul Khaiza, Wan Ghazali Wan Syamimee, Mohd Ismail Asmahan, Wan Ab Hamid Wan Zuraida
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.
Int Sch Res Notices. 2014 Oct 29;2014:275194. doi: 10.1155/2014/275194. eCollection 2014.
Objectives. The study was conducted to determine the correlation of ICAM-1, VCAM-1, and anti-C1q antibody levels with SLE disease activity index (SLEDAI) and standard SLE disease activity immunological markers (anti-dsDNA and sera C3 and C4). Study Design. This was a cross-sectional study. Materials and Methods. Blood samples were obtained from 95 SLE patients (45 active SLE and 50 nonactive SLE) and 50 controls. The subjects were assessed using SLEDAI and score of more than five is determined as having active SLE. The sera were tested for serum ICAM-1, VCAM-1, and anti-C1q (ELISA), anti-dsDNA (CLIFT), serum C3, and serum C4 (immunonephelometry). Results. Anti-dsDNA and anti-C1q antibody showed good positive correlations with SLEDAI (r = 0.529, P < 0.001 and r = 0.559, P < 0.001, resp.). VCAM-1 and sera C3 and C4 showed fair correlation with SLEDAI (r = 0.294, P = 0.004; r = -0.312, P = 0.002; and r = -0.382, P < 0.001, resp.). ICAM-1 level showed no significant correlation with SLEDAI (P = 0.062). There were significant correlations of VCAM-1 and anti-C1q antibody with anti-dsDNA (r = 0.226, P = 0.006 and r = 0.511, P < 0.001, resp.). VCAM-1 showed poor inverse correlation with serum C3 (r = -0.183, P = 0.028) and fair inverse correlation with serum C4 (r = -0.251, P = 0.002). Anti-C1q antibody demonstrated fair inverse correlation with both sera C3 and C4 (r = -0.420, P ≤ 0.001 and r = -0.398, P < 0.001, resp.). However, ICAM-1 showed no significant correlation with anti-dsDNA and sera C3 and C4 (P = 0.259, P = 0.626 and P = 0.338, resp.). Conclusions. The serum levels of anti-C1q antibody in SLE patients showed the best correlation with the SLEDAI and standard immunological tests for SLE disease activity. These data support that anti-C1q antibody is a useful marker for monitoring SLE global disease activity. The potential of VCAM-1 needs further confirmation.
目的。本研究旨在确定细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和抗C1q抗体水平与系统性红斑狼疮疾病活动指数（SLEDAI）以及系统性红斑狼疮疾病活动的标准免疫标志物（抗双链DNA和血清C3及C4）之间的相关性。研究设计。这是一项横断面研究。材料与方法。从95例系统性红斑狼疮患者（45例活动期系统性红斑狼疮患者和50例非活动期系统性红斑狼疮患者）和50例对照者中采集血样。使用SLEDAI对受试者进行评估，得分超过5分被判定为患有活动期系统性红斑狼疮。检测血清中的ICAM-1、VCAM-1和抗C1q（酶联免疫吸附测定法）、抗双链DNA（间接免疫荧光法）、血清C3和血清C4（免疫比浊法）。结果。抗双链DNA和抗C1q抗体与SLEDAI呈良好的正相关（r分别为0.529，P<0.001和r为0.559，P<0.001）。VCAM-1以及血清C3和C4与SLEDAI呈中等程度相关（r分别为0.294，P=0.004；r=-0.312，P=0.002；r=-0.382，P<0.001）。ICAM-1水平与SLEDAI无显著相关性（P=0.062）。VCAM-1和抗C1q抗体与抗双链DNA有显著相关性（r分别为0.226，P=0.006和r为0.511，P<0.001）。VCAM-1与血清C3呈弱负相关（r=-0.183，P=0.028），与血清C4呈中等程度负相关（r=-0.251，P=0.002）。抗C1q抗体与血清C3和C4均呈中等程度负相关（r分别为-0.420，P≤0.001和r为-0.398，P<0.001）。然而，ICAM-1与抗双链DNA以及血清C3和C4无显著相关性（P分别为0.259、P=0.626和P=0.338）。结论。系统性红斑狼疮患者血清中的抗C1q抗体水平与SLEDAI以及系统性红斑狼疮疾病活动的标准免疫检测显示出最佳相关性。这些数据支持抗C1q抗体是监测系统性红斑狼疮整体疾病活动的有用标志物。VCAM-1的潜力需要进一步证实。
