Gateva A, Assyov Y, Tsakova A, Kamenov Z
Department of Internal Medicine, Medical University - Sofia, Clinic of Endocrinology, University Hospital "Alexandrovska", Sofia, Bulgaria.
Department of Clinical Laboratory and Immunology, Medical University - Sofia, Central Clinical Laboratory, University Hospital "Alexandrovska", Sofia, Bulgaria.
Exp Clin Endocrinol Diabetes. 2016 Jul;124(7):444-7. doi: 10.1055/s-0042-108447. Epub 2016 Jun 29.
Paraoxonase-1 (PON1) is an extracellular esterase contributing to the anti-atherogenic and anti-inflammatory properties of HDL and has a protective role in the progression of atherosclerosis. The aim of the present study was to compare serum paraoxonase-1 levels between obese subjects with prediabetes and normal glucose tolerance.
In this study were included 76 patients with mean age 50.7±10.7 years, divided into 2 age and BMI-matched groups - group 1 with obesity without glycemic disturbances (n=38) and group 2 with prediabetes (n=38). Oral glucose tolerance test (OGTT) with measurement of immunoreactive insulin was performed in all participants and levels of PON1 were measured using ELISA method.
There was only a tendency towards higher PON1 levels in patients with prediabetes compared to controls. However, individuals with insulin resistance had significantly lower levels of PON1 compared to those without. The levels of PON1 showed a negative correlation with the blood glucose on 120th minute of OGTT, fasting IRI, HOMA index, BMI, WSR, WHR and particularly with waist circumference and positive with HDL-cholesterol.
The levels of PON1 are similar in patients with and wthout prediabetes, but are significantly decreased in patients with insulin resistance and correlate with anthropometric markers of visceral adiposity.
对氧磷酶-1（PON1）是一种细胞外酯酶，有助于高密度脂蛋白的抗动脉粥样硬化和抗炎特性，在动脉粥样硬化进展中起保护作用。本研究的目的是比较患有糖尿病前期的肥胖受试者与糖耐量正常者之间的血清对氧磷酶-1水平。
本研究纳入了76例平均年龄为50.7±10.7岁的患者，分为2个年龄和体重指数匹配的组——第1组为无血糖紊乱的肥胖患者（n = 38），第2组为患有糖尿病前期的患者（n = 38）。所有参与者均进行了口服葡萄糖耐量试验（OGTT）并测定免疫反应性胰岛素，采用酶联免疫吸附测定法（ELISA）测量PON1水平。
与对照组相比，糖尿病前期患者的PON1水平仅呈现出升高的趋势。然而，与无胰岛素抵抗的个体相比，有胰岛素抵抗的个体的PON1水平显著更低。PON1水平与OGTT第120分钟时的血糖、空腹胰岛素释放指数（IRI）、稳态模型评估胰岛素抵抗指数（HOMA指数）、体重指数（BMI）、腰围身高比（WSR）、腰臀比（WHR），尤其是腰围呈负相关，与高密度脂蛋白胆固醇呈正相关。
患有和未患有糖尿病前期的患者的PON1水平相似，但胰岛素抵抗患者的PON1水平显著降低，且与内脏肥胖的人体测量指标相关。
