Lamers W H, Geerts W J, Moorman A F, Dottin R P
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Anat Embryol (Berl). 1989;179(4):387-93. doi: 10.1007/BF00305065.
The distribution pattern of creatine kinase (EC 2.7.3.2) isozymes in developing chicken heart was studied by immunohistochemistry. Creatine kinase M, which is absent from adult heart, is transiently expressed between 4 and 11 days of incubation. During that period, numerous muscular cells in the roof and septum of the atrium, in the interventricular septum and on top of the trabeculae cordis and at the rim of the outflow tract stain strongly with a polyclonal antibody that is specific for the M subunit. In the ventricle and outflow tract, the M-positive cells are found mainly subendocardially and in the right half, at the transition of conducting and working myocytes. Creatine kinase B, which is the predominant adult isozyme, is initially expressed to a high concentration in a small group of disperse myocardial cells in upstream part of the inflow tract. When compared to the expression pattern of cardiac myosin heavy chains, the observed creatine kinase expression pattern suggests that M-positive cells are mainly found in areas that participate in the formation of cardiac conductive tissue, whereas B-positive cells are first found in areas that are involved in the generation of cardiac rhythm.
通过免疫组织化学研究了发育中的鸡心脏中肌酸激酶（EC 2.7.3.2）同工酶的分布模式。肌酸激酶M在成年心脏中不存在，在孵化4至11天期间短暂表达。在此期间，心房顶部和隔膜、室间隔、小梁嵴顶部以及流出道边缘的许多肌肉细胞，用针对M亚基的多克隆抗体染色强烈。在心室和流出道中，M阳性细胞主要在内皮下以及传导性和工作性心肌细胞过渡处的右半部分被发现。肌酸激酶B是成年期主要的同工酶，最初在流入道上游一小群分散的心肌细胞中高浓度表达。与心肌肌球蛋白重链的表达模式相比，观察到的肌酸激酶表达模式表明，M阳性细胞主要存在于参与心脏传导组织形成的区域，而B阳性细胞首先出现在参与心脏节律产生的区域。
