Kim Dong Hyun, Singh Priyanka, Farh Mohamed El-Agamy, Kim Yeon-Ju, Nguyen Ngoc-Lan, Lee Hyun A, Yang Deok-Chun
Department of Oriental Medicine Biotechnology, Ginseng Bank, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-Si, Gyeonggi-do, 446-701, Republic of Korea.
Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-Si, Gyeonggi-do, 446-701, Republic of Korea.
Antonie Van Leeuwenhoek. 2016 Sep;109(9):1199-208. doi: 10.1007/s10482-016-0720-7. Epub 2016 Jun 29.
A novel bacterial strain, designated DCY106(T), was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY106(T) is Gram-negative, yellow-pigmented, non-flagellate, motile, non-spore-forming, rod-shaped, and strictly aerobic. The strain grows optimally at 25-30 °C and pH 6.5-7.5. Phylogenetically, strain DCY106(T) is closely related to Flavobacterium arsenitoxidans KCTC 22507(T) (98.41 %), followed by Flavobacterium cutihirudini LMG 26922(T) (97.67 %), Flavobacterium nitrogenifigens LMG 28694(T) (97.59 %), Flexibacter auranticus LMG 3987(T) (97.38 %), Flavobacterium defluvi KCTC 12612(T) (97.21 %) and Flavobacterium chilense LMG 26360(T) (97.05 %). The 16S rRNA gene sequence similarities to all other Flavobacterium species were below 97 %. The DNA G+C content of strain DCY106(T) is 34.2 mol% and the DNA-DNA relatedness between strain DCY106(T) and F. cutihirudini LMG 26922(T), F. auranticus LMG 3987(T), F. defluvi KCTC 12612(T) and F. chilense LMG 26360(T) were below 40.0 %. The menaquinone of the type MK-6 was found to be the predominant respiratory quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidylserine, two unidentified aminolipids (APL1, APL6) and one unidentified lipid L2. C15:0, iso-C15:0 and summed feature 3 (iso-C15:0 2OH/C16:1 ω7c) were identified as the major fatty acids present in DCY106(T). The results of physiological and biochemical tests allowed strain DCY106(T) to be differentiated phenotypically from other recognized species belonging to the genus Flavobacterium. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Flavobacterium panacis sp. nov. is proposed with the type strain designated as DCY106(T) (= JCM 31468(T)= KCTC 42747(T)).
从韩国高敞地区人参（Panax ginseng）根际土壤中分离出一株新的细菌菌株，命名为DCY106(T)。菌株DCY106(T)为革兰氏阴性菌，黄色色素沉着，无鞭毛，具运动性，不形成芽孢，杆状，严格需氧。该菌株在25 - 30°C和pH 6.5 - 7.5条件下生长最佳。系统发育分析表明，菌株DCY106(T)与砷氧化黄杆菌Flavobacterium arsenitoxidans KCTC 22507(T)密切相关（相似度为98.41%），其次是切氏黄杆菌Flavobacterium cutihirudini LMG 26922(T)（97.67%）、固氮黄杆菌Flavobacterium nitrogenifigens LMG 28694(T)（97.59%）、橙色屈挠杆菌Flexibacter auranticus LMG 3987(T)（97.38%）、河流黄杆菌Flavobacterium defluvi KCTC 12612(T)（97.21%）和智利黄杆菌Flavobacterium chilense LMG 26360(T)（97.05%）。其与所有其他黄杆菌属物种的16S rRNA基因序列相似度均低于97%。菌株DCY106(T)的DNA G + C含量为34.2 mol%，菌株DCY106(T)与切氏黄杆菌LMG 26922(T)、橙色屈挠杆菌LMG 3987(T)、河流黄杆菌KCTC 12612(T)和智利黄杆菌LMG 26360(T)之间的DNA - DNA相关性均低于40.0%。发现主要呼吸醌为MK - 6型甲基萘醌。主要极性脂类鉴定为磷脂酰乙醇胺、磷脂酰丝氨酸、两种未鉴定的氨基脂类（APL1、APL6）和一种未鉴定的脂类L2。C15:0、异C15:0和总和特征3（异C15:0 2OH/C16:1 ω7c）被鉴定为DCY106(T)中存在的主要脂肪酸。生理生化测试结果使菌株DCY106(T)在表型上与黄杆菌属其他已确认物种区分开来。因此，建议将新分离的生物体代表一个新物种，提议将其命名为参黄杆菌Flavobacterium panacis sp. nov.，模式菌株指定为DCY106(T)（= JCM 31468(T)= KCTC 42747(T)）。
