Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4289-4293. doi: 10.1099/ijs.0.053819-0. Epub 2013 Aug 2.
A novel bacterial strain, designated DCY54(T), was isolated from a field cultivated with ginseng in Yongin, Republic of Korea. Cells were Gram-reaction-negative, yellow-pigmented, rod-shaped, non-spore-forming, and strictly aerobic. They were motile by gliding and produced flexirubin-type pigments. Growth occurred optimally at 25-30 °C, at pH 5.0-7.0 and in the presence of 0-1 % NaCl. The 16S rRNA sequence analysis demonstrated that strain DCY54(T) was most closely related to Flavobacterium defluvii EMB117(T) (96.9 %). The only isoprenoid quinone of strain DCY 54(T) was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The major cellular fatty acids (>15 %) were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The DNA G+C content was 33.3 mol%. Phylogenetic inference and phenotypic data supported affiliation of strain DCY54(T) to the genus Flavobacterium. Several physiological and biochemical tests differentiated strain DCY54(T) from the species of the genus Flavobacterium with validly published names. On the basis of data from a polyphasic study, strain DCY54(T) represents a novel species of the genus Flavobacterium for which the name Flavobacterium ginsengisoli sp. nov. is proposed. The type strain is DCY54(T) ( = KCTC 23318(T) = JCM 17336(T)).
一株新的细菌菌株，命名为 DCY54(T)，从韩国龙仁市人参种植地的土壤中分离得到。细胞革兰氏反应阴性，呈黄色，杆状，不形成孢子，严格需氧。它们通过滑行运动，产生黄质素型色素。在 25-30°C、pH5.0-7.0 和 0-1%NaCl 的条件下生长最佳。16S rRNA 序列分析表明，菌株 DCY54(T)与 Flavobacterium defluvii EMB117(T)（96.9%）最为密切相关。菌株 DCY54(T)的唯一类异戊二烯醌是甲萘醌-6（MK-6），主要极性脂是磷脂酰乙醇胺、一种未鉴定的胺脂和一种未鉴定的脂。主要细胞脂肪酸（>15%）为异-C15:0、特征性 3 组分（包含 C16:1ω7c 和/或异-C15:0 2-OH）和 C16:0。DNA G+C 含量为 33.3mol%。系统发育推断和表型数据支持菌株 DCY54(T)属于黄杆菌属。几种生理生化试验将菌株 DCY54(T)与已发表有效名的黄杆菌属种区分开来。基于多相研究的数据，菌株 DCY54(T)代表黄杆菌属的一个新种，现提议命名为人参黄杆菌（Flavobacterium ginsengisoli）sp. nov.。模式菌株为 DCY54(T)（ = KCTC 23318(T) = JCM 17336(T)）。
