Bu Ji-Hye, Cha Chang-Jun
Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Feb;68(2):616-622. doi: 10.1099/ijsem.0.002553. Epub 2018 Jan 5.
A Gram-staining-negative bacterial strain, designated CJ42, was isolated from ginseng soil in Anseong, Republic of Korea. Cells were rod-shaped, yellow-pigmented, aerobic and devoid of flagella but showed gliding motility. Strain CJ42 grew optimally at 30 °C and pH 7.0 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain CJ42 belonged to the genus Flavobacterium within the family Flavobacteriaceae and was most closely related to Flavobacterium tistrianum KCTC 42679 (97.3 % similarity). Flexirubin-type pigments were produced. The only respiratory quinone was menaquinone 6. The predominant polar lipids were phosphatidylethanolamine, an unknown aminolipid and two unknown lipids. The major fatty acids of strain CJ42 were iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The G+C content of the genomic DNA was 30.7 mol%. Based on the polyphasic analyses, strain CJ42 represents a novel species in the genus Flavobacterium, for which the name Flavobacteriumfoetidum sp. nov. is proposed. The type strain is CJ42 (=KACC 19302=JCM 32085).
从韩国安城人参土壤中分离出一株革兰氏染色阴性细菌菌株，命名为CJ42。细胞呈杆状，黄色色素沉着，好氧，无鞭毛，但具有滑行运动能力。菌株CJ42在胰蛋白胨大豆琼脂上，于30°C、pH 7.0且无氯化钠的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株CJ42属于黄杆菌科黄杆菌属，与蒂氏黄杆菌KCTC 42679关系最为密切（相似度为97.3%）。产生了flexirubin型色素。唯一的呼吸醌是甲基萘醌6。主要极性脂类为磷脂酰乙醇胺、一种未知氨基脂类和两种未知脂类。菌株CJ42的主要脂肪酸为异C15:0和总和特征3（包含C16:1ω7c和/或C16:1ω6c）。基因组DNA的G+C含量为30.7mol%。基于多相分析，菌株CJ42代表黄杆菌属中的一个新物种，为此提出名为恶臭黄杆菌（Flavobacterium foetidum sp. nov.）。模式菌株为CJ42（=KACC 19302=JCM 32085）。
