Lin Ze, Xiong Yuan, Hu Yiqiang, Chen Lang, Panayi Adriana C, Xue Hang, Zhou Wu, Yan Chenchen, Hu Liangcong, Xie Xudong, Sun Yun, Mi Bobin, Liu Guohui
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China.
Front Cell Dev Biol. 2021 Sep 29;9:730362. doi: 10.3389/fcell.2021.730362. eCollection 2021.
Polydatin (POL) is a natural active compound found in with reported anti-oxidant and antiviral effects. With the aging population there has been a stark increase in the prevalence of osteoporosis (OP), rendering it an imposing public health issue. The potential effect of POL as a therapy for OP remains unclear. Therefore, we sought to investigate the therapeutic effect of POL in OP and to elucidate the underlying signaling mechanisms in its regulatory process. The POL-targeted genes interaction network was constructed using the Search Tool for Interacting Chemicals (STITCH) database, and the shared Kyoto Encyclopedia of Genes and Genomes (KEGG). Pathways involved in OP and POL-targeted genes were identified. Quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were performed to evaluate the osteogenic genes and the phosphorylation level in pre-osteoblastic cells. In addition, ALP and alizarin red staining was used to test the effect of POL on extracellular matrix mineralization. Twenty-seven KEGG pathways shared between POL-related genes and OP were identified. signaling was identified as a potential key mechanism. results highlighted a definitive anti-OP effect of POL. The phosphorylation levels of signaling, including α, , and , were significantly decreased in this regulatory process. Our results suggest that POL has a promising therapeutic effect in OP. signaling may be the underlying mechanism in this effect, providing a novel sight in discovering new drugs for OP.
虎杖苷（POL）是一种天然活性化合物，存在于多种植物中，具有抗氧化和抗病毒作用。随着人口老龄化，骨质疏松症（OP）的患病率急剧上升，使其成为一个严峻的公共卫生问题。POL作为一种治疗OP的潜在效果仍不清楚。因此，我们试图研究POL对OP的治疗效果，并阐明其调节过程中的潜在信号机制。使用相互作用化学物质搜索工具（STITCH）数据库和共享的京都基因与基因组百科全书（KEGG）构建了POL靶向基因相互作用网络。确定了参与OP和POL靶向基因的途径。进行了定量实时PCR（qRT-PCR）和酶联免疫吸附测定（ELISA）以评估前成骨细胞中的成骨基因和磷酸化水平。此外，使用碱性磷酸酶（ALP）和茜素红染色来测试POL对细胞外基质矿化的影响。确定了POL相关基因和OP之间共有的27条KEGG途径。信号传导被确定为一种潜在的关键机制。结果突出了POL明确的抗OP作用。在这个调节过程中，包括α、β和γ在内的信号传导的磷酸化水平显著降低。我们的结果表明，POL在OP中具有有前景的治疗效果。信号传导可能是这种作用的潜在机制，为发现OP的新药提供了新的视角。
