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Utility of Whole-Genome Sequencing in Characterizing Acinetobacter Epidemiology and Analyzing Hospital Outbreaks.全基因组测序在描述不动杆菌流行病学及分析医院感染暴发中的应用
J Clin Microbiol. 2016 Mar;54(3):593-612. doi: 10.1128/JCM.01818-15. Epub 2015 Dec 23.
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Core Genome Multilocus Sequence Typing Scheme for High- Resolution Typing of Enterococcus faecium.用于屎肠球菌高分辨率分型的核心基因组多位点序列分型方案
J Clin Microbiol. 2015 Dec;53(12):3788-97. doi: 10.1128/JCM.01946-15.
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A Year of Infection in the Intensive Care Unit: Prospective Whole Genome Sequencing of Bacterial Clinical Isolates Reveals Cryptic Transmissions and Novel Microbiota.重症监护病房的感染一年：细菌临床分离株的前瞻性全基因组测序揭示隐匿传播和新型微生物群
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Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole-Genome Sequence-Based Typing of Listeria monocytogenes.定义和评估用于单核细胞增生李斯特菌全基因组序列分型的核心基因组多位点序列分型方案
J Clin Microbiol. 2015 Sep;53(9):2869-76. doi: 10.1128/JCM.01193-15. Epub 2015 Jul 1.
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Ensuring backwards compatibility: traditional genotyping efforts in the era of whole genome sequencing.确保向后兼容性：全基因组测序时代的传统基因分型工作。
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Acinetobacter baumannii: evolution of antimicrobial resistance-treatment options.鲍曼不动杆菌：抗菌药物耐药性的演变及治疗选择
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Application of whole-genome sequencing for bacterial strain typing in molecular epidemiology.全基因组测序在分子流行病学细菌菌株分型中的应用。
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Longitudinal epidemiology of multidrug-resistant (MDR) Acinetobacter species in a tertiary care hospital.多药耐药（MDR）不动杆菌属在一家三级医院的纵向流行病学研究。
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Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology.采用快速下一代测序技术对德国肠出血性大肠杆菌 O104:H4 暴发进行前瞻性基因组特征分析。
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全基因组测序阐明鲍曼不动杆菌医院感染聚集性病例的流行病学特征。

Whole-Genome Sequencing Elucidates Epidemiology of Nosocomial Clusters of Acinetobacter baumannii.

作者信息

Willems Stefanie, Kampmeier Stefanie, Bletz Stefan, Kossow Annelene, Köck Robin, Kipp Frank, Mellmann Alexander

机构信息

Institute of Hygiene, University Hospital Muenster, Muenster, Germany.

Institute of Medical Microbiology, University Hospital Muenster, Muenster, Germany.

出版信息

J Clin Microbiol. 2016 Sep;54(9):2391-4. doi: 10.1128/JCM.00721-16. Epub 2016 Jun 29.

DOI:10.1128/JCM.00721-16
PMID:27358465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5005500/
Abstract

We characterized two epidemiologically similar Acinetobacter baumannii clusters from two separate intensive care units (ICU) using core genome multilocus sequence typing. Clonal spread was confirmed in ICU-1 (12 of 14 isolates shared genotypes); in ICU-2, all genotypes (13 isolates) were diverse, thus excluding transmissions and enabling adequate infection control measures.

摘要

我们使用核心基因组多位点序列分型对来自两个不同重症监护病房（ICU）的两个流行病学特征相似的鲍曼不动杆菌菌群进行了特征分析。在ICU-1中证实存在克隆传播（14株分离株中有12株共享基因型）；在ICU-2中，所有基因型（13株分离株）各不相同，因此排除了传播并得以采取适当的感染控制措施。