重新审视 MLST：基于基因的细菌基因组学研究方法。
MLST revisited: the gene-by-gene approach to bacterial genomics.
机构信息
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
出版信息
Nat Rev Microbiol. 2013 Oct;11(10):728-36. doi: 10.1038/nrmicro3093. Epub 2013 Sep 2.
Abstract
Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.
摘要
多位点序列分型（MLST）于 1998 年被提出，作为一种基于序列的可移植方法，用于鉴定细菌之间的克隆关系。如今，在微生物学的全基因组时代，对细菌基因型变异进行系统、标准化描述的需求仍然是当务之急。在这里，为了满足这一需求，我们借鉴了 MLST 和 16S rRNA 基因测序的成功经验，提出了一种层次化的逐基因方法，反映了功能和进化关系，并对细菌进行了从域到株的分类。我们使用在线平台（如细菌分离基因组序列数据库（BIGSdb））的基于基因的分型方法允许对全基因组序列数据进行可扩展的组织和分析。
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