Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany; Sustainable Chemistry, Institute of Sustainable Environmental Chemistry, Leuphana University of Lüneburg, Scharnhorststr. 1, C13, 21335 Lüneburg, Germany.
Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Bioresour Technol. 2016 Oct;218:167-73. doi: 10.1016/j.biortech.2016.06.078. Epub 2016 Jun 21.
In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121°C for 30min in presence of 0.18molL(-1) H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (gL(-1)) glucose (20-30), xylose (15-25), sucrose (5-11) and arabinose (0.7-10). Fermentations were carried out at laboratory (2L) and pilot (50L) scales in presence of 10gL(-1) yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78gg(-1), respectively. The productivity was 4.02gL(-1)h(-1). Downstream processing resulted in a pure formulation containing 937gL(-1)l(+)-lactic acid with an optical purity of 99.7%.
在这项研究中，使用木质纤维素残余物咖啡渣作为碳源，利用凝结芽孢杆菌进行发酵生产 l(+)-乳酸。在 121°C 下用 0.18mol/L H2SO4 进行热化学处理 30min 后，采用 Accellerase 1500 进行酶解，得到富含碳的水解产物。使用两种具有相似生物质组成的不同咖啡渣材料，但水解产物中的糖浓度存在差异。主要糖为（gL(-1)）葡萄糖（20-30）、木糖（15-25）、蔗糖（5-11）和阿拉伯糖（0.7-10）。在存在 10gL(-1)酵母提取物的情况下，在实验室（2L）和中试（50L）规模下进行发酵。在中试规模下，碳利用率和每克消耗糖的乳酸产率分别为 94.65%和 0.78gg(-1)，生产率为 4.02gL(-1)h(-1)。下游处理得到了一种含有 937gL(-1)l(+)-乳酸的纯制剂，光学纯度为 99.7%。
