Wei Kang, Zhang Yazhen, Wu Liyun, Li Hailin, Ruan Li, Bai Peixian, Zhang Chengcai, Zhang Fen, Xu Liyi, Wang Liyuan, Cheng Hao
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
Plant Physiol Biochem. 2016 Oct;107:310-318. doi: 10.1016/j.plaphy.2016.06.022. Epub 2016 Jun 17.
Purple shoot tea attributing to the high anthocyanin accumulation is of great interest for its wide health benefits. To better understand potential mechanisms involved in purple buds and leaves formation in tea plants, we performed transcriptome analysis of six green or purple shoot tea individuals from a F1 population using the Illumina sequencing method. Totally 292 million RNA-Seq reads were obtained and assembled into 112,233 unigenes, with an average length of 759 bp and an N50 of 1081 bp. Moreover, totally 2193 unigenes showed significant differences in expression levels between green and purple tea samples, with 1143 up- and 1050 down-regulated in the purple teas. Further real time PCR analysis confirmed RNA-Seq results. Our study identified 28 differentially expressed transcriptional factors and A CsMYB gene was found to be highly similar to AtPAP1 in Arabidopsis. Further analysis of differentially expressed genes involved in anthocyanin biosynthesis and transportation showed that the late biosynthetic genes and genes involved in anthocyanin transportation were largely affected but the early biosynthetic genes were less or none affected. Overall, the identification of a large number of differentially expressed genes offers a global view of the potential mechanisms associated with purple buds and leaves formation, which will facilitate molecular breeding in tea plants.
富含高花青素的紫芽茶因其广泛的健康益处而备受关注。为了更好地了解茶树紫芽和紫叶形成的潜在机制，我们使用Illumina测序方法对一个F1群体中的六个绿色或紫芽茶个体进行了转录组分析。共获得2.92亿条RNA-Seq reads，并组装成112,233个单基因，平均长度为759 bp，N50为1081 bp。此外，共有2193个单基因在绿色和紫色茶样品之间的表达水平存在显著差异，其中1143个在紫茶中上调，1050个下调。进一步的实时PCR分析证实了RNA-Seq结果。我们的研究鉴定出28个差异表达的转录因子，并且发现一个CsMYB基因与拟南芥中的AtPAP1高度相似。对参与花青素生物合成和运输的差异表达基因的进一步分析表明，后期生物合成基因和参与花青素运输的基因受到很大影响，但早期生物合成基因受到的影响较小或没有受到影响。总体而言，大量差异表达基因的鉴定为与紫芽和紫叶形成相关的潜在机制提供了一个全局视图，这将有助于茶树的分子育种。
