Wang Yueqi, Jin Ji-Qiang, Zhang Rui, He Mengdi, Wang Liubin, Mao Zhuozhuo, Gan Min, Wu Liyun, Chen Liang, Wang Liyuan, Wei Kang
Hortic Res. 2024 Jul 10;11(9):uhae191. doi: 10.1093/hr/uhae191. eCollection 2024 Sep.
Purple tea, rich in anthocyanins, has a variety of health benefits and is attracting global interest. However, the regulation mechanism of anthocyanin in purple tea populations has not been extensively studied. In this experiment, RNA-seq, BSA-seq, and BSR-seq were performed using 30 individuals with extreme colors (dark-purple and green) in an population of 'Zijuan' and 'Jinxuan'. The results show that 459 genes were differentially expressed in purple and green leaves, among which genes involved in the anthocyanin synthesis and transport pathway, such as , , , , , , and were highly expressed in purple leaves. Moreover, there were multiple SNP/InDel variation sites on chromosomes 2 and 14 of the tea plant, as identified by BSA-seq. The integrated analysis identified two highly expressed genes ( and ) with SNP/InDel site variations in the purple tea plants. By silencing leaves, we proved that could positively regulate anthocyanin accumulation and expression of related structural genes in tea plants. A 181-bp InDel in the promoter was also found to be co-segregating with leaf color. The results of this study provide a theoretical reference for the molecular mechanism of anthocyanin accumulation in purple tea plants and contribute to the creation of new tea cultivars with high anthocyanin content.
紫茶富含花青素，具有多种健康益处，正吸引着全球的关注。然而，紫茶群体中花青素的调控机制尚未得到广泛研究。在本实验中，利用“紫娟”和“金萱”群体中30个颜色极端（深紫色和绿色）的个体进行了RNA测序、混池分离群体测序（BSA-seq）和混池分组分析法测序（BSR-seq）。结果表明，紫色叶和绿色叶中有459个基因差异表达，其中参与花青素合成和转运途径的基因，如[具体基因名称未给出]在紫色叶中高表达。此外，通过BSA-seq鉴定出茶树2号和14号染色体上有多个单核苷酸多态性/插入缺失（SNP/InDel）变异位点。综合分析确定了紫茶植株中两个具有SNP/InDel位点变异的高表达基因（[具体基因名称未给出]）。通过叶片沉默，我们证明[具体基因名称未给出]可以正向调控茶树中花青素的积累和相关结构基因的表达。还发现[具体基因名称未给出]启动子中的一个181bp插入缺失与叶色共分离。本研究结果为紫茶植株花青素积累的分子机制提供了理论参考，并有助于培育高花青素含量的新茶树品种。
