Petrezsélyová Silvia, López-Malo María, Canadell David, Roque Alicia, Serra-Cardona Albert, Marqués M Carmen, Vilaprinyó Ester, Alves Rui, Yenush Lynne, Ariño Joaquín
Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
PLoS One. 2016 Jun 30;11(6):e0158424. doi: 10.1371/journal.pone.0158424. eCollection 2016.
Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase.
在出芽酵母酿酒酵母中，Ena1钠-ATP酶的表达调控是适应高盐和/或碱性pH胁迫的关键事件。ENA1的表达受多种信号通路的控制，包括由钙可调节蛋白磷酸酶钙调神经磷酸酶及其下游转录因子Crz1介导的信号通路。我们在此展示了一项关于Ena1在环境碱化时表达的定量研究，并分析了Crz1对该反应的贡献。实验数据和数学模型证实了ENA1启动子中存在两个应激反应性Crz1结合位点，并估计Crz1对ENA1启动子早期反应的贡献约为60%。这些模型表明存在第二个具有相似动力学的输入，这可能是由高pH诱导的Snf1激酶激活介导的。
