Mendizabal I, Pascual-Ahuir A, Serrano R, de Larrinoa I F
Universidad del País Vasco, Facultad de Ciencias Químicas, Departamento de Química Aplicada, San Sebastián, Spain.
Mol Genet Genomics. 2001 Jul;265(5):801-11. doi: 10.1007/s004380100474.
In Saccharomyces cerevisiae the transcription of the ENA1 gene is modulated by multiple transduction pathways that respond to osmotic, ionic and nutrient stresses. We have investigated the molecular mechanisms involved in ENA1 induction by the calcium-calcineurin-activated transcription factor Crzl/Tcn1. We found in the ENA1 promoter a calcium-responsive, Crzl-dependent upstream activating region (UASENA1) located between -713 bp and 826 bp relative to the translation start. This region contains two separate control elements: the upstream element (5'-GAATGGCTG-3') between -813 and -821 binds Crzlp with lower affinity and mostly contributes to basal ENA1 expression, whereas the downstream element (5'-GGGTGGCTG-3') between 727 and 719 binds Crz1p with higher affinity and is a major determinant of the induction response to calcium.
在酿酒酵母中，ENA1基因的转录受多种转导途径调控，这些途径对渗透压、离子和营养胁迫作出反应。我们研究了钙-钙调磷酸酶激活的转录因子Crzl/Tcn1诱导ENA1表达所涉及的分子机制。我们在ENA1启动子中发现了一个钙响应、Crzl依赖的上游激活区域（UASENA1），相对于翻译起始位点，其位于-713 bp至-826 bp之间。该区域包含两个独立的控制元件：位于-813至-821之间的上游元件（5'-GAATGGCTG-3'）以较低亲和力结合Crzlp，主要促进ENA1的基础表达；而位于-727至-719之间的下游元件（5'-GGGTGGCTG-3'）以较高亲和力结合Crz1p，是钙诱导反应的主要决定因素。
