使用含有血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的透明质酸水凝胶支架对大鼠进行自体移植后卵巢组织功能和发情周期的恢复
Restoration of ovarian tissue function and estrous cycle in rat after autotransplantation using hyaluronic acid hydrogel scaffold containing VEGF and bFGF.
作者信息
Tavana Somayeh, Valojerdi Mojtaba Rezazadeh, Azarnia Mahnaz, Shahverdi Abdolhossein
机构信息
a Department of Embryology , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran .
b Department of Animal Biology , Faculty of Biological Sciences, Kharazmi University , Tehran , Iran , and.
出版信息
Growth Factors. 2016 Aug;34(3-4):97-106. doi: 10.1080/08977194.2016.1194835. Epub 2016 Jun 30.
This study investigates the effect of hyaluronic acid (HA) containing VEGF and bFGF on restoration of ovarian function after ovarian autotransplantation. Twenty-four rats were randomly divided into three groups for ovarian autotransplantation: group A (ovaries without HA, VEGF and bFGF), group B (ovaries encapsulated with HA) and group C (ovaries encapsulated with HA containing VEGF and bFGF). The grafts were assessed using vaginal smears, histological, hormonal, and the genes expression analysis. The duration of first estrous cycle was shorter in group C than in group A (p < 0.01). The mean number of primordial follicles was protected in group C. The level of estradiol was higher in group A than in group C (p < 0.01). The expression level of Cellular-Myelocytomatosis (C-Myc) in group C was lower than in group B (p < 0.05). HA containing VEGF and bFGF can ensure follicular survival, decrease apoptosis and recover ovarian function after auto-transplantation.
本研究探讨含血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的透明质酸（HA）对卵巢自体移植后卵巢功能恢复的影响。将24只大鼠随机分为三组进行卵巢自体移植：A组（不含HA、VEGF和bFGF的卵巢）、B组（用HA包裹的卵巢）和C组（用含VEGF和bFGF的HA包裹的卵巢）。通过阴道涂片、组织学、激素及基因表达分析对移植物进行评估。C组首次发情周期的持续时间比A组短（p<0.01）。C组原始卵泡的平均数量得到了保护。A组雌二醇水平高于C组（p<0.01）。C组细胞髓细胞瘤（C-Myc）的表达水平低于B组（p<0.05）。含VEGF和bFGF的HA可确保自体移植后卵泡存活、减少细胞凋亡并恢复卵巢功能。
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