Rahman Mohammad A, Barger Jennifer F, Lovat Francesca, Gao Min, Otterson Gregory A, Nana-Sinkam Patrick
Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
Oncotarget. 2016 Aug 23;7(34):54852-54866. doi: 10.18632/oncotarget.10243.
Exosomes, a subgroup of extracellular vesicles (EVs), have been shown to serve as a conduit for the exchange of genetic information between cells. Exosomes are released from all types of cells but in abundance from cancer cells. The contents of exosomes consist of proteins and genetic material (mRNA, DNA and miRNA) from the cell of origin. In this study, we examined the effects of exosomes derived from human lung cancer serum and both highly metastatic and non-metastatic cells on recipient human bronchial epithelial cells (HBECs). We found that exosomes derived from highly metastatic lung cancer cells and human late stage lung cancer serum induced vimentin expression, and epithelial to mesenchymal transition (EMT) in HBECs. Exosomes derived from highly metastatic cancer cells as well as late stage lung cancer serum induce migration, invasion and proliferation in non-cancerous recipient cells. Our results suggest that cancer derived exosomes could be a potential mediator of EMT in the recipient cells.
外泌体是细胞外囊泡（EVs）的一个亚群，已被证明可作为细胞间遗传信息交换的一种媒介。外泌体由所有类型的细胞释放，但癌细胞释放的外泌体数量丰富。外泌体的内容物包括来自起源细胞的蛋白质和遗传物质（mRNA、DNA和miRNA）。在本研究中，我们检测了源自人肺癌血清以及高转移性和非转移性细胞的外泌体对人支气管上皮细胞（HBECs）的影响。我们发现，源自高转移性肺癌细胞和人晚期肺癌血清的外泌体可诱导HBECs中波形蛋白的表达以及上皮-间质转化（EMT）。源自高转移性癌细胞以及晚期肺癌血清的外泌体可诱导非癌受体细胞的迁移、侵袭和增殖。我们的结果表明，癌症来源的外泌体可能是受体细胞中EMT的潜在介质。
