Abouel-Nour Mohamed F, El-Shewehy Dina Magdy M, Hamada Shadia F, Morsy Tosson A
J Egypt Soc Parasitol. 2016 Apr;46(1):185-200. doi: 10.12816/0026164.
Cryptosporidiosis parvum is a zoonotic protozoan parasite infects intestinal epithelial cells of man and animals causing a major health problem. This study was oriented to evaluate the protective and curative capacity of garlic, ginger and mirazid in comparison with metronidazole drug (commercially known) against Cryptosporidium in experimental mice. Male Swiss Albino mice experimentally infected with C. parvum were treated with medicinal plants extracts (Ginger, Mirazid, and Garlic) as compared to chemical drug Metronidazole. Importantly, C. parvum-infected mice treated with ginger, Mirazid, garlic and metronidazole showed a complete elimination in shedding oocysts by 9th day PI. The reduction and elimination of shedding oocysts in response to the treatments might be attributable to a direct effect on parasite growth in intestines, sexual phases production and/or the formation of oocysts. The results were evaluated histopathological examination of ideum section of control mice (uninfected, untreated) displayed normal architecture of the villi. Examiination of infected mice ileum section (infected, untreated) displayed histopathological alterations from uninfected groups. Examination of ileum section prepared from mice treated with garlic, ginger, mirazid, and metronidazole displayed histopathological alterations from that of the control groups, and showed marked histologic correction in the pattern with the four regimes used in comparison to control mice. Garlic successfully eradicated oocysts of infected mice from stool and intestine. Supplementation of ginger to infected mice markedly corrected elevation in the inflammatory risk factors and implied its potential antioxidant, anti-inflammatory and immunomodulatory capabilities. Infected mice treated with ginger, mirazid, garlic and metronidazole showed significant symptomatic improvements during treatment.
微小隐孢子虫是一种人畜共患的原生动物寄生虫，可感染人和动物的肠道上皮细胞，引发严重的健康问题。本研究旨在评估大蒜、生姜和咪康唑与甲硝唑药物（商业上已知）相比，对实验小鼠体内隐孢子虫的保护和治疗能力。将经实验感染微小隐孢子虫的雄性瑞士白化小鼠用药用植物提取物（生姜、咪康唑和大蒜）进行治疗，并与化学药物甲硝唑进行比较。重要的是，用生姜、咪康唑、大蒜和甲硝唑治疗的微小隐孢子虫感染小鼠在感染后第9天粪便中卵囊排泄完全消除。治疗后卵囊排泄的减少和消除可能归因于对肠道内寄生虫生长、有性阶段产生和/或卵囊形成的直接影响。通过组织病理学检查对结果进行评估，未感染、未治疗的对照小鼠回肠切片显示绒毛结构正常。感染小鼠回肠切片（感染、未治疗）的检查显示与未感染组相比存在组织病理学改变。对用大蒜、生姜、咪康唑和甲硝唑治疗的小鼠制备的回肠切片进行检查，显示与对照组相比存在组织病理学改变，并且与对照小鼠相比，在使用的四种治疗方案中组织学模式有明显改善。大蒜成功地从粪便和肠道中清除了感染小鼠的卵囊。给感染小鼠补充生姜显著纠正了炎症风险因素的升高，并暗示了其潜在的抗氧化、抗炎和免疫调节能力。用生姜、咪康唑、大蒜和甲硝唑治疗的感染小鼠在治疗期间症状有显著改善。
