Vishwanatha Udupi, Guruprasad Kanive P, Gopinath Puthiya M, Acharya Raviraj V, Prasanna Bokkasa V, Nayak Jayakrishna, Ganesh Rajeshwari, Rao Jayalaxmi, Shree Rashmi, Anchan Suchitra, Raghu Kothanahalli S, Joshi Manjunath B, Paladhi Puspendu, Varier Panniampilly M, Muraleedharan Kollath, Muraleedharan Thrikovil S, Satyamoorthy Kapaettu
School of Life Sciences, Manipal University, Planetarium Complex, Manipal 576 104, Karnataka, India.
Department of Medicine, Kasturba Medical College Hospital, Manipal University, Manipal 576 104, Karnataka, India.
J Ethnopharmacol. 2016 Sep 15;191:387-397. doi: 10.1016/j.jep.2016.06.062. Epub 2016 Jun 27.
Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals.
Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed.
Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups.
Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.
在印度传统阿育吠陀医学体系中，余甘子（Phyllanthus emblica）制剂阿马拉吉·拉萨亚纳（Amalaki rasayana）用于老年人的健康生活。其生物学效应及其机制尚未完全了解。由于DNA修复能力下降是衰老的标志，我们测试了阿马拉吉·拉萨亚纳对健康老年人公认的DNA修复活性的影响。
新鲜制备阿马拉吉·拉萨亚纳，按照传统文本严格要求，将健康的老年随机志愿者分为两组，分别给予拉萨亚纳或安慰剂，为期45天。在服用拉萨亚纳之前、服用期间以及停用拉萨亚纳45天后，对外周血单核细胞中的DNA修复进行分析。通过荧光分析基于DNA解旋程度检测紫外线C诱导的DNA链断裂修复（DSBR），通过流式细胞术检测核苷酸切除修复（NER），通过缺口填充法检测组成型碱基切除修复（BER）。
服用阿马拉吉·拉萨亚纳能稳定维持/增强老年人的DSBR。未观察到不良副作用。此外，不同体重指数的受试者表现出不同的DNA链断裂修复能力。两组之间在NER和BER过程中未观察到非计划DNA合成的变化。
老年人摄入阿马拉吉·拉萨亚纳可稳定维持DNA链断裂修复，且无毒性作用。然而，核苷酸切除修复和碱基切除修复活性未发生变化。结果表明有必要进一步研究阿马拉吉·拉萨亚纳对DSBR活性的影响。
