Molecular detection of vancomycin and methicillin resistance in isolated from food processing environments.

is a well-known foodborne pathogen. The aim of this study was to investigate the presence of isolated from serving utensils in food processing environments in Mymensingh city, Bangladesh and to determine their antibiogram and resistance determinants. A total of 120 environmental samples were collected from different food settings. Isolation and identification were conducted using conventional biochemical tests. Molecular identification of isolates and detection of methicillin and vancomycin resistance were done using primer-specific polymerase chain reaction (PCR) targeting , , , and genes. Antibiotic sensitivity tests were performed, and resistance genes were also detected by amplifying , genes. Among the 120 samples, 81 (67.5%) were positive for spp. and 41 (50.62%) were positive for the -gene. Among the 41 isolates, 5 (12.20%) were positive for , but none were positive for the gene. A total of 12.2% of the isolates were -positive, of which 4 isolates (9.76%) were also positive for the gene. Antibiotic sensitivity testing revealed that all isolates (100%) from hotel samples were sensitive to ciprofloxacin and chloramphenicol, 90.32% were sensitive to doxycycline, and 80.65% were sensitive to streptomycin. Conversely, all isolates (100%) were resistant to ampicillin, and 29.03% were resistant to vancomycin. All isolates obtained from non-hotel samples were susceptible to chloramphenicol, ceftriaxone, ciprofloxacin, doxycycline, meropenem, and vancomycin; however, 40% of isolates were resistant to novobiocin. Among the hotel isolates, 29 (93.55%) of the ampicillin-resistant isolates harbored the gene while 5 (55.55%) of the vancomycin-resistant isolates harbored the gene. Four of the five positive isolates were also positive for the gene. The presence of methicillin-resistant (MRSA) which is also vancomycin-resistant in food processing environments is a threat to public health. This is the first report on the molecular detection of methicillin and vancomycin-resistant isolated from food processing environments in Bangladesh.