Sakkas Hercules, Gousia Panagiota, Economou Vangelis, Sakkas Vassilios, Petsios Stefanos, Papadopoulou Chrissanthy
Department of Microbiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Ioannina, Greece.
Department of Hygiene and Technology of Food of Animal Origin, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Intercult Ethnopharmacol. 2016 May 30;5(3):212-8. doi: 10.5455/jice.20160331064446. eCollection 2016 Jun-Aug.
AIM/BACKGROUND: The emergence of drug-resistant pathogens has drawn attention on medicinal plants for potential antimicrobial properties. The objective of the present study was the investigation of the antimicrobial activity of five plant essential oils on multidrug resistant Gram-negative bacteria.
Basil, chamomile blue, origanum, thyme, and tea tree oil were tested against clinical isolates of Acinetobacter baumannii (n = 6), Escherichia coli (n = 4), Klebsiella pneumoniae (n = 7), and Pseudomonas aeruginosa (n = 5) using the broth macrodilution method.
The tested essential oils produced variable antibacterial effect, while Chamomile blue oil demonstrated no antibacterial activity. Origanum, Thyme, and Basil oils were ineffective on P. aeruginosa isolates. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration values ranged from 0.12% to 1.50% (v/v) for tea tree oil, 0.25-4% (v/v) for origanum and thyme oil, 0.50% to >4% for basil oil and >4% for chamomile blue oil. Compared to literature data on reference strains, the reported MIC values were different by 2SD, denoting less successful antimicrobial activity against multidrug resistant isolates.
The antimicrobial activities of the essential oils are influenced by the strain origin (wild, reference, drug sensitive, or resistant) and it should be taken into consideration whenever investigating the plants' potential for developing new antimicrobials.
目的/背景：耐药病原体的出现引发了人们对具有潜在抗菌特性的药用植物的关注。本研究的目的是调查五种植物精油对多重耐药革兰氏阴性菌的抗菌活性。
采用肉汤稀释法，对罗勒、蓝甘菊、牛至、百里香和茶树精油针对鲍曼不动杆菌（n = 6）、大肠杆菌（n = 4）、肺炎克雷伯菌（n = 7）和铜绿假单胞菌（n = 5）的临床分离株进行了测试。
所测试的精油产生了不同的抗菌效果，而蓝甘菊精油没有显示出抗菌活性。牛至、百里香和罗勒精油对铜绿假单胞菌分离株无效。茶树精油的最低抑菌浓度（MIC）和最低杀菌浓度值范围为0.12%至1.50%（v/v），牛至和百里香精油为0.25 - 4%（v/v），罗勒精油为0.50%至>4%，蓝甘菊精油为>4%。与参考菌株的文献数据相比，报告的MIC值相差2个标准差，表明对多重耐药分离株的抗菌活性较差。
精油的抗菌活性受菌株来源（野生、参考、药物敏感或耐药）的影响，在研究植物开发新型抗菌剂的潜力时应予以考虑。
