Sohrab Sayed Sartaj, Yasir Muhammad, El-Kafrawy Sherif Ali, Abbas Ayman T, Mousa Magdi Ali Ahmed, Bakhashwain Ahmed A
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Post Box No. 80216, Jeddah, 21589 Saudi Arabia.
Faculty of Metrology and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Horticulture, Assiut University, Assiut, Egypt.
Virusdisease. 2016 Jun;27(2):145-53. doi: 10.1007/s13337-016-0308-x. Epub 2016 Mar 4.
Tomato is an important vegetable crop and its production is adversely affected by leaf curl disease caused by begomovirus. Leaf curl disease is a serious concern for tomato crops caused by begomovirus in Jeddah, Kingdom of Saudi Arabia. Tomato leaf curl disease has been shown to be mainly caused either by tomato leaf curl Sudan virus or tomato yellow leaf curl virus as well as tomato leaf curl Oman virus. Many tomato plants infected with monopartite begomoviruses were also found to harbor a symptom enhancing betasatellites. Here we report the association of tomato leaf curl Sudan virus causing leaf curl disease of tomato in Jeddah, Kingdom of Saudi Arabia. The complete genome sequence analysis showed highest (99.9 %) identity with tomato leaf curl Sudan virus causing leaf curl disease in Arabian Peninsula. In phylogenetic relationships analysis, the identified virus formed closest cluster with tomato leaf curl Sudan virus. In recombination analysis study, the major parent was identified as tomato leaf curl Sudan virus. Findings of this study strongly supports the associated virus is a variant of tomato leaf curl Sudan virus causing disease in Sudan, Yemen and Arabian Peninsula. The betasatellites sequence analysis showed highest identity (99.8 %) with tomato leaf curl betasatellites-Amaranthus-Jeddah. The phylogenetic analysis result based on betasatellites formed closed cluster with tomato yellow leaf curl Oman betasatellites. The importance of these findings and occurrence of begomovirus in new geographic regions causing leaf curl disease of tomato in Jeddah, Kingdom of Saudi Arabia are discussed.
番茄是一种重要的蔬菜作物，其产量受到由双生病毒引起的卷叶病的不利影响。在沙特阿拉伯王国吉达，由双生病毒引起的番茄卷叶病是番茄作物面临的一个严重问题。已表明番茄卷叶病主要由番茄卷叶苏丹病毒、番茄黄化曲叶病毒以及番茄卷叶阿曼病毒引起。还发现许多感染单分体双生病毒的番茄植株携带一种增强症状的卫星DNA分子。在此，我们报告了在沙特阿拉伯王国吉达引起番茄卷叶病的番茄卷叶苏丹病毒的关联情况。完整基因组序列分析显示，与在阿拉伯半岛引起卷叶病的番茄卷叶苏丹病毒具有最高（99.9%）的同一性。在系统发育关系分析中，鉴定出的病毒与番茄卷叶苏丹病毒形成了最紧密的聚类。在重组分析研究中，主要亲本被鉴定为番茄卷叶苏丹病毒。本研究结果有力地支持了所关联的病毒是在苏丹、也门和阿拉伯半岛引起病害的番茄卷叶苏丹病毒的一个变体。卫星DNA分子序列分析显示，与番茄卷叶卫星DNA分子 - 苋属 - 吉达具有最高同一性（99.8%）。基于卫星DNA分子的系统发育分析结果与番茄黄化曲叶阿曼卫星DNA分子形成了紧密聚类。讨论了这些发现的重要性以及双生病毒在新地理区域出现导致沙特阿拉伯王国吉达番茄卷叶病的情况。
