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由分生组织特异性KNAT1启动子驱动过表达FT的拟南芥植物数据集。

Dataset of Arabidopsis plants that overexpress FT driven by a meristem-specific KNAT1 promoter.

作者信息

Duplat-Bermúdez L, Ruiz-Medrano R, Landsman D, Mariño-Ramírez L, Xoconostle-Cázares B

机构信息

Departamento de Biotecnología y Bioingeniería; Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Av. IPN 2508 San Pedro Zacatenco, 07360 Mexico City, Mexico.

Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, United States of America, Bethesda, MD, USA.

出版信息

Data Brief. 2016 Jun 8;8:520-8. doi: 10.1016/j.dib.2016.06.002. eCollection 2016 Sep.

DOI:10.1016/j.dib.2016.06.002
PMID:27366785
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4919726/
Abstract

In this dataset we integrated figures comparing leaf number and rosette diameter in three Arabidopsis FT overexpressor lines (AtFTOE) driven by KNAT1 promoter, "A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis" [5], vs Wild Type (WT) Arabidopsis plats. Also, presented in the tables are some transcriptomic data obtained by RNA-seq Illumina HiSeq from rosette leaves of Arabidopsis plants of AtFTOE 2.1 line vs WT with accession numbers SRR2094583 and SRR2094587 for AtFTOE replicates 1-3 and AtWT for control replicates 1-2 respectively. Raw data of paired-end sequences are located in the public repository of the National Center for Biotechnology Information of the National Library of Medicine, National Institutes of Health, United States of America, Bethesda, MD, USA as Sequence Read Archive (SRA). Performed analyses of differential expression genes are visualized by Mapman and presented in figures. "Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering" [2], described the interpretation and discussion of the obtained data.

摘要

在该数据集中,我们整合了一些图表,用于比较由KNAT1启动子驱动的三个拟南芥FT过表达系(AtFTOE)(“由拟南芥STM基因编码的同源域蛋白KNOTTED类的一个成员”[5])与野生型(WT)拟南芥植株的叶片数量和莲座直径。此外,表格中还呈现了一些转录组数据,这些数据是通过RNA-seq Illumina HiSeq从AtFTOE 2.1系拟南芥植株的莲座叶中获得的,与WT相比,AtFTOE重复1-3的登录号为SRR2094583和SRR2094587,对照重复1-2的登录号为AtWT。双端序列的原始数据位于美国国立医学图书馆、美国国立卫生研究院国家生物技术信息中心的公共储存库中,作为序列读取存档(SRA)。通过Mapman对差异表达基因进行分析,并在图中呈现。“由诱导早花的分生组织特异性启动子驱动的拟南芥开花位点T过表达的转录组分析”[2]描述了对所得数据的解释和讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/d8cec9e64612/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/2ec231bbabd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/7614c76a7066/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/d8cec9e64612/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/2ec231bbabd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/7614c76a7066/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c9a/4919726/d8cec9e64612/gr3.jpg

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本文引用的文献

1
Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering.由分生组织特异性启动子驱动的过表达开花位点T的拟南芥转录组分析,该启动子可诱导早花。
Gene. 2016 Aug 10;587(2):120-31. doi: 10.1016/j.gene.2016.04.060. Epub 2016 May 3.
2
Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants.方案:优化水培生长系统,以进行拟南芥和其他植物的营养和生理分析。
Plant Methods. 2013 Feb 5;9(1):4. doi: 10.1186/1746-4811-9-4.
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Fast gapped-read alignment with Bowtie 2.
快速缺口读对准与 Bowtie 2。
Nat Methods. 2012 Mar 4;9(4):357-9. doi: 10.1038/nmeth.1923.
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Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks.RNA-seq 实验中使用 TopHat 和 Cufflinks 的差异基因和转录本表达分析。
Nat Protoc. 2012 Mar 1;7(3):562-78. doi: 10.1038/nprot.2012.016.
5
TopHat: discovering splice junctions with RNA-Seq.TopHat:利用RNA测序发现剪接接头
Bioinformatics. 2009 May 1;25(9):1105-11. doi: 10.1093/bioinformatics/btp120. Epub 2009 Mar 16.
6
MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes.MAPMAN:一种用户驱动的工具,用于将基因组数据集显示在代谢途径和其他生物过程的图表上。
Plant J. 2004 Mar;37(6):914-39. doi: 10.1111/j.1365-313x.2004.02016.x.
7
A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis.一种由拟南芥STM基因编码的同源异型域蛋白的结瘤类成员。
Nature. 1996 Jan 4;379(6560):66-9. doi: 10.1038/379066a0.