Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
Anal Chem. 2016 Aug 2;88(15):7627-32. doi: 10.1021/acs.analchem.6b01355. Epub 2016 Jul 13.
A portable, microfluidic blood plasma separation device is presented featuring a constriction-expansion design, which produces 100.0% purity for undiluted blood at 9% yield. This level of purity represents an improvement of at least 1 order of magnitude with increased yield compared to that achieved previously using passive separation. The system features high flow rates, 5-30 μL/min plasma collection, with minimal clogging and biofouling. The simple, portable blood plasma separation design is hand-driven and can easily be incorporated with microfluidic or laboratory scale diagnostic assays. The separation system was applied to a paper-based diagnostic test for malaria that produced an amplified color change in the presence of Plasmodium falciparum histidine-rich protein 2 at a concentration well below clinical relevancy for undiluted whole blood.
一种便携式微流控血浆分离装置，采用收缩-扩张设计，可实现未稀释血液 9%的产率和 100.0%的纯度。与以前使用被动分离技术相比，这种纯度水平提高了至少 1 个数量级，产率也有所提高。该系统具有高流速，5-30 μL/min 的血浆采集量，几乎不会发生堵塞和生物污垢。这种简单、便携式的血浆分离设计是手动驱动的，可以很容易地与微流控或实验室规模的诊断检测相结合。该分离系统应用于一种基于纸张的疟疾诊断测试，在低于临床相关浓度的全血中，当存在恶性疟原虫富含组氨酸蛋白 2 时，会产生放大的颜色变化。
