Gaye O, Diouf M, Dansokho E F, McLaughlin G, Diallo S
Service de Parasitologie, Faculté de Médecine, UCAD, Dakar, Sénégal.
Parasite. 1998 Jun;5(2):189-92. doi: 10.1051/parasite/1998052189.
A battery of sixty-six blood samples from Senegal was analysed by the ParaSight F test, the ICT Malaria PF and the Malaria IgG CELISA. These three assays detect the histidine rich protein 2 antigen of Plasmodium falciparum. Thick smear microscopy was used as the reference method. Sensitivity, specificity, predictive positive and negative values were respectively 89%, 100%, 100%, 88% for the ICT; 86%, 93%, 94%, 85% for the paraSight and 88%, 87%, 88%, 87% for the Malaria IgG CELISA. The three assays failed to detect two positive samples with P. ovale and P. malariae. Assays were also compared with regard to the expense of equipment and reagents and speed and ease of use. The rapid ICT and ParaSight F test can be performed with minimal training and may be specially useful in areas where P. falciparum is the predominant malaria species, in epidemic malaria regions, and where skilled microscopy is not readily available.
对来自塞内加尔的66份血样进行了ParaSight F检测、ICT疟疾恶性疟检测和疟疾IgG捕获酶联免疫吸附测定。这三种检测方法均可检测恶性疟原虫富含组氨酸的蛋白2抗原。厚涂片显微镜检查用作参考方法。ICT的敏感性、特异性、阳性预测值和阴性预测值分别为89%、100%、100%、88%；ParaSight的相应数值分别为86%、93%、94%、85%；疟疾IgG捕获酶联免疫吸附测定的相应数值分别为88%、87%、88%、87%。这三种检测方法均未能检测出两份卵形疟和三日疟阳性样本。还对检测方法在设备和试剂成本以及速度和易用性方面进行了比较。快速ICT和ParaSight F检测只需极少培训即可进行，在以恶性疟原虫为主要疟原虫种类的地区、疟疾流行地区以及缺乏熟练显微镜检查人员的地区可能特别有用。
