Rogers Christopher S
Exemplar Genetics, Coralville, Iowa.
Curr Protoc Hum Genet. 2016 Jul 1;90:15.9.1-15.9.14. doi: 10.1002/cphg.18.
Animal models are an important resource for studying human diseases. Genetically engineered mice are the most commonly used species and have made significant contributions to our understanding of basic biology, disease mechanisms, and drug development. However, they often fail to recreate important aspects of human diseases and thus can have limited utility as translational research tools. Developing disease models in species more similar to humans may provide a better setting in which to study disease pathogenesis and test new treatments. This unit provides an overview of the history of genetically engineered large animals and the techniques that have made their development possible. Factors to consider when planning a large animal model, including choice of species, type of modification and methodology, characterization, production methods, and regulatory compliance, are also covered. © 2016 by John Wiley & Sons, Inc.
动物模型是研究人类疾病的重要资源。基因工程小鼠是最常用的物种，对我们理解基础生物学、疾病机制和药物开发做出了重大贡献。然而，它们常常无法重现人类疾病的重要方面，因此作为转化研究工具的效用可能有限。在与人类更相似的物种中开发疾病模型，可能为研究疾病发病机制和测试新疗法提供更好的环境。本单元概述了基因工程大型动物的历史以及使其得以发展的技术。还涵盖了规划大型动物模型时需要考虑的因素，包括物种选择、修饰类型和方法、特征鉴定、生产方法以及法规遵循情况。© 2016约翰威立国际出版公司。
