Bui Anh Thy, Beyler Maryline, Liao Yuan-Yuan, Grichine Alexei, Duperray Alain, Mulatier Jean-Christophe, Guennic Boris Le, Andraud Chantal, Maury Olivier, Tripier Raphaël
Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
Université de Brest, UMR-CNRS 6521, UFR des Sciences et Techniques 6 avenue Victor le Gorgeu, C.S. 93837, 29238, Brest Cedex 3, France.
Inorg Chem. 2016 Jul 18;55(14):7020-5. doi: 10.1021/acs.inorgchem.6b00891. Epub 2016 Jul 1.
An original cationic water-soluble cyclen-based Eu(III) complex EuL(1) featuring a chromophore-functionalized antenna to increase the two-photon (2P) absorption properties was synthesized. The photophysical properties were thoroughly studied in various solvents and rationalized with the help of theoretical calculations. The complex exhibits an optimized 2P absorption cross section. Finally, 2P microscopy imaging experiments on living T24 human cancer cells highlighted the spontaneous internalization and the biological stability of this 2P bioprobe in vitro. Macrocyclic-based antennas open new perspectives for future optimization of the photophysical properties and allows envisaging the design of Eu, Tb, Yb, and Sm bioprobes. This result also opens the way for the design of functional two-photon Ln complexes able to monitor intracellular physicochemical parameters.
合成了一种基于阳离子水溶性轮环的新型铕（III）配合物[EuL(1)]⁺，其具有发色团功能化天线以增强双光子（2P）吸收特性。在各种溶剂中对其光物理性质进行了深入研究，并借助理论计算进行了合理分析。该配合物展现出优化的2P吸收截面。最后，对活的T24人癌细胞进行的2P显微镜成像实验突出了这种2P生物探针在体外的自发内化和生物稳定性。基于大环的天线为未来光物理性质的优化开辟了新的前景，并有助于设想铕、铽、镱和钐生物探针的设计。这一结果也为设计能够监测细胞内物理化学参数的功能性双光子镧系配合物开辟了道路。
